The human bitter taste receptor hTAS2R39 is the primary receptor for the bitterness of theaflavins

Toyomi Yamazaki; Miki Sagisaka; Riko Ikeda; Toshiyuki Nakamura; Noriko Matsuda; Takeshi Ishii; Tsutomu Nakayama; Tatsuo Watanabe

doi:10.1080/09168451.2014.930326

The human bitter taste receptor hTAS2R39 is the primary receptor for the bitterness of theaflavins

Toyomi Yamazaki, Miki Sagisaka, Riko Ikeda, Toshiyuki Nakamura, Noriko Matsuda, Takeshi Ishii, Tsutomu Nakayama, Tatsuo Watanabe

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We purified several hundred mgs of four major theaflavins (theaflavin, theaflavin-3-O-gallate, theaflavin-3′-O-gallate, and theaflavin-3,3′-O-digallate). Among the 25 hTAS2Rs expressed in HEK293T cells, hTAS2R39 and hTAS2R14 were activated by theaflavins. Both hTAS2R39 and hTAS2R14 responded to theaflavin-3′-O-gallate. In addition, hTAS2R39 was activated by theaflavin and theaflavin-3,3′-O-gallate, but not by theaflavin-3-O-gallate. In contrast, hTAS2R14 responded to theaflavin-3-O-gallate.

Original language	English
Pages (from-to)	1753-1756
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	78
Issue number	10
DOIs	https://doi.org/10.1080/09168451.2014.930326
Publication status	Published - 2014
Externally published	Yes

Keywords

Human bitter taste receptor
Human bitter taste receptor 14 (hTAS2R14)
Human bitter taste receptor 39 (hTAS2R39)
Theaflavins

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2014.930326

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title = "The human bitter taste receptor hTAS2R39 is the primary receptor for the bitterness of theaflavins",

abstract = "We purified several hundred mgs of four major theaflavins (theaflavin, theaflavin-3-O-gallate, theaflavin-3′-O-gallate, and theaflavin-3,3′-O-digallate). Among the 25 hTAS2Rs expressed in HEK293T cells, hTAS2R39 and hTAS2R14 were activated by theaflavins. Both hTAS2R39 and hTAS2R14 responded to theaflavin-3′-O-gallate. In addition, hTAS2R39 was activated by theaflavin and theaflavin-3,3′-O-gallate, but not by theaflavin-3-O-gallate. In contrast, hTAS2R14 responded to theaflavin-3-O-gallate.",

keywords = "Human bitter taste receptor, Human bitter taste receptor 14 (hTAS2R14), Human bitter taste receptor 39 (hTAS2R39), Theaflavins",

author = "Toyomi Yamazaki and Miki Sagisaka and Riko Ikeda and Toshiyuki Nakamura and Noriko Matsuda and Takeshi Ishii and Tsutomu Nakayama and Tatsuo Watanabe",

note = "Funding Information: We thank Dr Takumi Misaka (University of Tokyo, Tokyo, Japan) and Dr Takashi Ueda (Nagoya City University, Nagoya, Japan) for providing the hTAS2R genes and the Ga16gust44 construct, respectively. This work was supported by the Shizuoka Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST. Publisher Copyright: {\textcopyright} 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.",

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doi = "10.1080/09168451.2014.930326",

language = "English",

volume = "78",

pages = "1753--1756",

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T1 - The human bitter taste receptor hTAS2R39 is the primary receptor for the bitterness of theaflavins

AU - Yamazaki, Toyomi

AU - Sagisaka, Miki

AU - Ikeda, Riko

AU - Nakamura, Toshiyuki

AU - Matsuda, Noriko

AU - Ishii, Takeshi

AU - Nakayama, Tsutomu

AU - Watanabe, Tatsuo

N1 - Funding Information: We thank Dr Takumi Misaka (University of Tokyo, Tokyo, Japan) and Dr Takashi Ueda (Nagoya City University, Nagoya, Japan) for providing the hTAS2R genes and the Ga16gust44 construct, respectively. This work was supported by the Shizuoka Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST. Publisher Copyright: © 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

PY - 2014

Y1 - 2014

N2 - We purified several hundred mgs of four major theaflavins (theaflavin, theaflavin-3-O-gallate, theaflavin-3′-O-gallate, and theaflavin-3,3′-O-digallate). Among the 25 hTAS2Rs expressed in HEK293T cells, hTAS2R39 and hTAS2R14 were activated by theaflavins. Both hTAS2R39 and hTAS2R14 responded to theaflavin-3′-O-gallate. In addition, hTAS2R39 was activated by theaflavin and theaflavin-3,3′-O-gallate, but not by theaflavin-3-O-gallate. In contrast, hTAS2R14 responded to theaflavin-3-O-gallate.

AB - We purified several hundred mgs of four major theaflavins (theaflavin, theaflavin-3-O-gallate, theaflavin-3′-O-gallate, and theaflavin-3,3′-O-digallate). Among the 25 hTAS2Rs expressed in HEK293T cells, hTAS2R39 and hTAS2R14 were activated by theaflavins. Both hTAS2R39 and hTAS2R14 responded to theaflavin-3′-O-gallate. In addition, hTAS2R39 was activated by theaflavin and theaflavin-3,3′-O-gallate, but not by theaflavin-3-O-gallate. In contrast, hTAS2R14 responded to theaflavin-3-O-gallate.

KW - Human bitter taste receptor

KW - Human bitter taste receptor 14 (hTAS2R14)

KW - Human bitter taste receptor 39 (hTAS2R39)

KW - Theaflavins

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The human bitter taste receptor hTAS2R39 is the primary receptor for the bitterness of theaflavins

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Keywords

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