The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel

Yusuke Nagai; Hidenori Yokoi; Keiko Kaihara; Keiji Naruse

doi:10.1016/j.biomaterials.2011.10.049

The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel

Yusuke Nagai, Hidenori Yokoi, Keiko Kaihara, Keiji Naruse

Research output: Contribution to journal › Article › peer-review

84 Citations (Scopus)

Abstract

The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment. In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 ± 1.5 times during 8 days of incubation; mean ± SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 ± 0.2 times; mean ± SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.

Original language	English
Pages (from-to)	1044-1051
Number of pages	8
Journal	Biomaterials
Volume	33
Issue number	4
DOIs	https://doi.org/10.1016/j.biomaterials.2011.10.049
Publication status	Published - Feb 2012

Keywords

Cell proliferation
Hydrogel
Mechanical strain
Scaffold
Self-assembly

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2011.10.049

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title = "The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel",

abstract = "The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment. In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 ± 1.5 times during 8 days of incubation; mean ± SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 ± 0.2 times; mean ± SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.",

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author = "Yusuke Nagai and Hidenori Yokoi and Keiko Kaihara and Keiji Naruse",

note = "Funding Information: We thank Ms. Masumi Furutani for her efficient technical support in obtaining the TEM images and Dr. Ken Takahashi for the helpful discussions on the experimental details. We are indebted to Dr. Yasuyuki Ishida at Chubu University for generously allowing us use of a MALDI-TOF mass spectrometer. This work was partially supported by Grant-in-Aid for Scientific Research of Priority Areas, 17076006 provided by the Ministry of Education, Culture, Sports, Sciences, and Technology of Japan (to K.N.), and Challenging Exploratory Research, 23650264 provided by the Japanese Society for the Promotion of Science (to K.N.). Y.N. and H.Y. were supported by Menicon Co., Ltd. , Japan. ",

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AU - Naruse, Keiji

N1 - Funding Information: We thank Ms. Masumi Furutani for her efficient technical support in obtaining the TEM images and Dr. Ken Takahashi for the helpful discussions on the experimental details. We are indebted to Dr. Yasuyuki Ishida at Chubu University for generously allowing us use of a MALDI-TOF mass spectrometer. This work was partially supported by Grant-in-Aid for Scientific Research of Priority Areas, 17076006 provided by the Ministry of Education, Culture, Sports, Sciences, and Technology of Japan (to K.N.), and Challenging Exploratory Research, 23650264 provided by the Japanese Society for the Promotion of Science (to K.N.). Y.N. and H.Y. were supported by Menicon Co., Ltd. , Japan.

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N2 - The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment. In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 ± 1.5 times during 8 days of incubation; mean ± SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 ± 0.2 times; mean ± SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.

AB - The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment. In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 ± 1.5 times during 8 days of incubation; mean ± SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 ± 0.2 times; mean ± SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.

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The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel

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Keywords

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