TY - JOUR
T1 - The Opsin 3/Teleost multiple tissue opsin system
T2 - mRNA localization in the retina and brain of medaka (Oryzias latipes)
AU - Sato, Keita
AU - Khine Nwe Nwe, K. N.N.
AU - Ohuchi, Hideyo
N1 - Funding Information:
The authors thank Prof. Satoru Miyaishi for permitting us to use his DNA sequencing facility. Confocal microscopy was performed using a facility of Central Research Laboratory, Okayama University Medical School. This work was supported by a Grant-in-Aid for Scientific Research 17K15159 (to Keita Sato) from the Japan Society for the Promotion of Science and a grant from the Astellas Pharma Inc. Research Support (to Hideyo Ohuchi).
Funding Information:
The authors thank Prof. Satoru Miyaishi for permitting us to use his DNA sequencing facility. Confocal microscopy was performed using a facility of Central Research Laboratory, Okayama University Medical School. This work was supported by a Grant‐in‐Aid for Scientific Research 17K15159 (to Keita Sato) from the Japan Society for the Promotion of Science and a grant from the Astellas Pharma Inc. Research Support (to Hideyo Ohuchi).
Publisher Copyright:
© 2021 Wiley Periodicals LLC
PY - 2021/7/1
Y1 - 2021/7/1
N2 - The photoreceptor protein, opsin, is one of the major components for vision and photoreceptive function in animals. Although many opsins have been discovered from animal genomes, only a few nonimage-forming functions mediated by opsins have been identified. Understanding the mRNA distribution of photoreceptor proteins is one crucial step in uncovering their photoreceptive function in animals. Here, we focus on the medaka fish (Oryzias latipes) Opsin 3 (Opn3)/Teleost multiple opsin (Tmt) system, which constitutes a separate phylogenetic group, having putative blue light photoreceptors for nonimage-forming functions. In medaka, there is one opn3 and five tmt-opsin orthologs. The expression pattern of the opn3/tmt-opsins in the retina and brain was investigated by in situ hybridization. mRNAs for opn3/tmt-opsins were distributed in the retinal ganglion cells as well as interneurons and specific brain nuclei. Specifically, hybridization signals were observed in the glutamate decarboxylase 1 (gad1)-expressing amacrine cells for opn3, tmt1a, tmt1b, and tmt2, in the caudal lobe of the cerebellum for tmt1b and tmt2, in the cranial nerve nuclei for opn3, tmt1a, tmt1b, tmt2, and in the rostral pars distalis (adenohypophysis) for opn3. These expression patterns suggest that blue light sensing in the fish retina and brain may be involved in the integration of visual inputs, vestibular function, somatosensation, motor outputs, and pituitary endocrine regulation.
AB - The photoreceptor protein, opsin, is one of the major components for vision and photoreceptive function in animals. Although many opsins have been discovered from animal genomes, only a few nonimage-forming functions mediated by opsins have been identified. Understanding the mRNA distribution of photoreceptor proteins is one crucial step in uncovering their photoreceptive function in animals. Here, we focus on the medaka fish (Oryzias latipes) Opsin 3 (Opn3)/Teleost multiple opsin (Tmt) system, which constitutes a separate phylogenetic group, having putative blue light photoreceptors for nonimage-forming functions. In medaka, there is one opn3 and five tmt-opsin orthologs. The expression pattern of the opn3/tmt-opsins in the retina and brain was investigated by in situ hybridization. mRNAs for opn3/tmt-opsins were distributed in the retinal ganglion cells as well as interneurons and specific brain nuclei. Specifically, hybridization signals were observed in the glutamate decarboxylase 1 (gad1)-expressing amacrine cells for opn3, tmt1a, tmt1b, and tmt2, in the caudal lobe of the cerebellum for tmt1b and tmt2, in the cranial nerve nuclei for opn3, tmt1a, tmt1b, tmt2, and in the rostral pars distalis (adenohypophysis) for opn3. These expression patterns suggest that blue light sensing in the fish retina and brain may be involved in the integration of visual inputs, vestibular function, somatosensation, motor outputs, and pituitary endocrine regulation.
KW - RRID: AB_840257
KW - RRID:AB_2336524
KW - RRID:AB_2339038
KW - RRID:AB_514497
KW - RRID:AB_514504
KW - RRID:SCR_004860
KW - RRID:SCR_005887
KW - RRID:SCR_010279
KW - RRID:SCR_018163 RRID:SCR_003070
KW - medaka
KW - nonimage-forming photoreception
KW - opn3
KW - opsin
KW - tmt-opsin
UR - http://www.scopus.com/inward/record.url?scp=85099950398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85099950398&partnerID=8YFLogxK
U2 - 10.1002/cne.25106
DO - 10.1002/cne.25106
M3 - Article
C2 - 33427299
AN - SCOPUS:85099950398
SN - 0021-9967
VL - 529
SP - 2484
EP - 2516
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 10
ER -