TY - JOUR
T1 - The photosystem i assembly apparatus consisting of Ycf3-Y3IP1 and Ycf4 modules
AU - Sreedhar, Nellaepalli
AU - Ozawa, Shin Ichiro
AU - Kuroda, Hiroshi
AU - Takahashi, Yuichiro
N1 - Funding Information:
This work was supported by the Japan Science and Technology Agency (JST), CREST, and JSPS KAKENHI grant numbers 16H06554
Funding Information:
We thank Prof. M. Goldschmidt-Clermont for the kind gift of cDNA library and pSL18 vector. We thank Prof. Kevin Redding for kindly providing the antibodies against PsaA. This work was supported by the Japan Science and Technology Agency (JST), CREST, and JSPS KAKENHI grant numbers 16H06554.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - In oxygenic photosynthesis, light energy is converted into redox energy by two photosystems (PSI and PSII). PSI forms one of the largest multiprotein complexes in thylakoid membranes consisting of a core complex, peripheral light-harvesting complexes (LHCIs) and cofactors. Although the high-resolution structure of the PSI-LHCI complex has been determined, the assembly process remains unclear due to the rapid nature of the assembly process. Here we show that two conserved chloroplast-encoded auxiliary factors, Ycf3 and Ycf4, form modules that mediate PSI assembly. The first module consists of the tetratricopeptide repeat protein Ycf3 and its interacting partner, Y3IP1, and mainly facilitates the assembly of reaction center subunits. The second module consists of oligomeric Ycf4 and facilitates the integration of peripheral PSI subunits and LHCIs into the PSI reaction center subcomplex. We reveal that these two modules are major mediators of the PSI-LHCI assembly process.
AB - In oxygenic photosynthesis, light energy is converted into redox energy by two photosystems (PSI and PSII). PSI forms one of the largest multiprotein complexes in thylakoid membranes consisting of a core complex, peripheral light-harvesting complexes (LHCIs) and cofactors. Although the high-resolution structure of the PSI-LHCI complex has been determined, the assembly process remains unclear due to the rapid nature of the assembly process. Here we show that two conserved chloroplast-encoded auxiliary factors, Ycf3 and Ycf4, form modules that mediate PSI assembly. The first module consists of the tetratricopeptide repeat protein Ycf3 and its interacting partner, Y3IP1, and mainly facilitates the assembly of reaction center subunits. The second module consists of oligomeric Ycf4 and facilitates the integration of peripheral PSI subunits and LHCIs into the PSI reaction center subcomplex. We reveal that these two modules are major mediators of the PSI-LHCI assembly process.
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U2 - 10.1038/s41467-018-04823-3
DO - 10.1038/s41467-018-04823-3
M3 - Article
C2 - 29934511
AN - SCOPUS:85048961324
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 2439
ER -