The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes

Le Thi Phuong; Lei Zhao; Aprilia Nur Fitrianti; Hidenori Matsui; Yoshiteru Noutoshi; Mikihiro Yamamoto; Yuki Ichinose; Tomonori Shiraishi; Kazuhiro Toyoda

doi:10.1007/s10327-019-00900-7

The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes

Le Thi Phuong, Lei Zhao, Aprilia Nur Fitrianti, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Saccharin and its parental compound probenazole (PBZ) are plant activators of effective defense responses to (hemi) biotrophic pathogens. Here, we demonstrate that pretreatment of wheat seedlings with saccharin or PBZ results in a significant reduction of powdery mildew caused by Blumeria graminis f. sp. tritici. Transcriptional analysis revealed the expression of 15 defense-related genes including PR genes, WCI genes, LOX, AOS, NPR1, PAL and WRKY genes in wheat seedlings exposed to either saccharin or PBZ. Moreover, the saccharin- and PBZ-enhanced expression of those genes during fungal infection further proved a close correlation with increased resistance in wheat.

Original language	English
Pages (from-to)	107-113
Number of pages	7
Journal	Journal of General Plant Pathology
Volume	86
Issue number	2
DOIs	https://doi.org/10.1007/s10327-019-00900-7
Publication status	Published - Mar 1 2020

Keywords

Blumeria graminis f. sp. tritici
Induced resistance
Plant activator
Probenazole (PBZ)
Saccharin
Wheat

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-019-00900-7

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title = "The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes",

abstract = "Saccharin and its parental compound probenazole (PBZ) are plant activators of effective defense responses to (hemi) biotrophic pathogens. Here, we demonstrate that pretreatment of wheat seedlings with saccharin or PBZ results in a significant reduction of powdery mildew caused by Blumeria graminis f. sp. tritici. Transcriptional analysis revealed the expression of 15 defense-related genes including PR genes, WCI genes, LOX, AOS, NPR1, PAL and WRKY genes in wheat seedlings exposed to either saccharin or PBZ. Moreover, the saccharin- and PBZ-enhanced expression of those genes during fungal infection further proved a close correlation with increased resistance in wheat.",

keywords = "Blumeria graminis f. sp. tritici, Induced resistance, Plant activator, Probenazole (PBZ), Saccharin, Wheat",

author = "Phuong, {Le Thi} and Lei Zhao and Fitrianti, {Aprilia Nur} and Hidenori Matsui and Yoshiteru Noutoshi and Mikihiro Yamamoto and Yuki Ichinose and Tomonori Shiraishi and Kazuhiro Toyoda",

note = "Funding Information: LTP thanks the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) for financial support during her doctoral program. This research was supported in part by the Grants-in-Aid for Scientific Research (18K05645) from the Japan Society for Promotion of Science (JSPS). Publisher Copyright: {\textcopyright} 2019, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.",

year = "2020",

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doi = "10.1007/s10327-019-00900-7",

language = "English",

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journal = "Journal of General Plant Pathology",

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T1 - The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes

AU - Phuong, Le Thi

AU - Zhao, Lei

AU - Fitrianti, Aprilia Nur

AU - Matsui, Hidenori

AU - Noutoshi, Yoshiteru

AU - Yamamoto, Mikihiro

AU - Ichinose, Yuki

AU - Shiraishi, Tomonori

AU - Toyoda, Kazuhiro

N1 - Funding Information: LTP thanks the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) for financial support during her doctoral program. This research was supported in part by the Grants-in-Aid for Scientific Research (18K05645) from the Japan Society for Promotion of Science (JSPS). Publisher Copyright: © 2019, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.

PY - 2020/3/1

Y1 - 2020/3/1

N2 - Saccharin and its parental compound probenazole (PBZ) are plant activators of effective defense responses to (hemi) biotrophic pathogens. Here, we demonstrate that pretreatment of wheat seedlings with saccharin or PBZ results in a significant reduction of powdery mildew caused by Blumeria graminis f. sp. tritici. Transcriptional analysis revealed the expression of 15 defense-related genes including PR genes, WCI genes, LOX, AOS, NPR1, PAL and WRKY genes in wheat seedlings exposed to either saccharin or PBZ. Moreover, the saccharin- and PBZ-enhanced expression of those genes during fungal infection further proved a close correlation with increased resistance in wheat.

AB - Saccharin and its parental compound probenazole (PBZ) are plant activators of effective defense responses to (hemi) biotrophic pathogens. Here, we demonstrate that pretreatment of wheat seedlings with saccharin or PBZ results in a significant reduction of powdery mildew caused by Blumeria graminis f. sp. tritici. Transcriptional analysis revealed the expression of 15 defense-related genes including PR genes, WCI genes, LOX, AOS, NPR1, PAL and WRKY genes in wheat seedlings exposed to either saccharin or PBZ. Moreover, the saccharin- and PBZ-enhanced expression of those genes during fungal infection further proved a close correlation with increased resistance in wheat.

KW - Blumeria graminis f. sp. tritici

KW - Induced resistance

KW - Plant activator

KW - Probenazole (PBZ)

KW - Saccharin

KW - Wheat

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The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes

Abstract

Keywords

ASJC Scopus subject areas

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