TY - JOUR
T1 - The presence of ABCG2-dependent side population cells in human periodontal ligaments
AU - Kawanabe, Noriaki
AU - Murakami, Kaoru
AU - Takano-Yamamoto, Teruko
N1 - Funding Information:
The present work was supported in part by a Grant-in-Aid for Scientific Research (to N.K. and to T.T.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2006/6/16
Y1 - 2006/6/16
N2 - Periodontal ligament (PDL) is capable of regenerating several types of periodontal tissues. However, the stem cells in PDL have been less well characterized. Recently, it has been represented that stem cells have a potent ability to exclude Hoechst dye, and cells possessing this phenotype are termed "side population (SP)." In addition, it is suggested that the SP cells are ubiquitously present in adult tissues, therefore, we investigated the presence of SP cells in PDL. Hoechst dye efflux assay showed that PDL cells include 3.9% SP cells. This SP profile disappeared in the presence of verapamil or reserpine. Only ABCG2-expressing cells were detected in PDL cells by flow cytometric analysis. Furthermore, RT-PCR demonstrated that ABCG2 mRNA expression remarkably correlated with PDL SP phenotype. Our findings demonstrate that PDL contains ABCG2-dependent SP cells. Hence, it is possible that this population could contribute to periodontal tissue regeneration as PDL stem cells.
AB - Periodontal ligament (PDL) is capable of regenerating several types of periodontal tissues. However, the stem cells in PDL have been less well characterized. Recently, it has been represented that stem cells have a potent ability to exclude Hoechst dye, and cells possessing this phenotype are termed "side population (SP)." In addition, it is suggested that the SP cells are ubiquitously present in adult tissues, therefore, we investigated the presence of SP cells in PDL. Hoechst dye efflux assay showed that PDL cells include 3.9% SP cells. This SP profile disappeared in the presence of verapamil or reserpine. Only ABCG2-expressing cells were detected in PDL cells by flow cytometric analysis. Furthermore, RT-PCR demonstrated that ABCG2 mRNA expression remarkably correlated with PDL SP phenotype. Our findings demonstrate that PDL contains ABCG2-dependent SP cells. Hence, it is possible that this population could contribute to periodontal tissue regeneration as PDL stem cells.
KW - ABCG2
KW - Human periodontal ligament
KW - Side population
KW - Stem cells
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U2 - 10.1016/j.bbrc.2006.03.237
DO - 10.1016/j.bbrc.2006.03.237
M3 - Article
C2 - 16650829
AN - SCOPUS:33646178915
SN - 0006-291X
VL - 344
SP - 1278
EP - 1283
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -