TY - JOUR
T1 - The presence of l-2,4-diaminobutyric acid decarboxylase activity in Vibrio species
T2 - A new biosynthetic pathway for 1,3-diaminopropane
AU - Yamamoto, Shigeo
AU - Suemoto, Yasuo
AU - Seito, Yumiko
AU - Nakao, Hiroshi
AU - Shinoda, Sumio
PY - 1986/7
Y1 - 1986/7
N2 - A new enzyme activity, which catalyzes decarboxylation of l-2,4-diaminobutyric acid (DABA) to yield 1,3-diaminopropane (DAP), has been found in dialyzed crude extracts prepared from Vibrio alginolyticus. The pH optimum for the activity was 8.0-8.5, and the enzyme showed a pyridoxal 5′-phosphate (PLP) requirement. Mg2+ caused about 30% stimulation in activity. The enzyme was active to only l-DABA among the diamino acids examined, and the Km value for l-DABA was 0.13 mM. Ammonium sulfate fractionation of a dialyzed crude extract followed by HPLC separation allowed us to conclude that this enzyme differed from the decarboxylase which occurs in Vibrio spp. to produce norspermidine (Nspd) for carboxynorspermidine (C-Nspd) having a moiety similar in structure to DABA. The same enzyme activity was detected in several other Vibrio species.
AB - A new enzyme activity, which catalyzes decarboxylation of l-2,4-diaminobutyric acid (DABA) to yield 1,3-diaminopropane (DAP), has been found in dialyzed crude extracts prepared from Vibrio alginolyticus. The pH optimum for the activity was 8.0-8.5, and the enzyme showed a pyridoxal 5′-phosphate (PLP) requirement. Mg2+ caused about 30% stimulation in activity. The enzyme was active to only l-DABA among the diamino acids examined, and the Km value for l-DABA was 0.13 mM. Ammonium sulfate fractionation of a dialyzed crude extract followed by HPLC separation allowed us to conclude that this enzyme differed from the decarboxylase which occurs in Vibrio spp. to produce norspermidine (Nspd) for carboxynorspermidine (C-Nspd) having a moiety similar in structure to DABA. The same enzyme activity was detected in several other Vibrio species.
KW - Pyridoxal 5′-phosphate-dependent enzyme
KW - polyamine
KW - precursor of norspermidine
UR - http://www.scopus.com/inward/record.url?scp=0022528941&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022528941&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1986.tb01545.x
DO - 10.1111/j.1574-6968.1986.tb01545.x
M3 - Article
AN - SCOPUS:0022528941
SN - 0378-1097
VL - 35
SP - 289
EP - 293
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2-3
ER -