TY - JOUR
T1 - The redox state of recombinant human serum albumin and its optimal concentration for mouse embryo culture
AU - Otsuki, Junko
AU - Nagai, Yasushi
AU - Matsuyama, Yukie
AU - Terada, Tomoyoshi
AU - Era, Seiichi
PY - 2013/2
Y1 - 2013/2
N2 - Albumin has multiple physiological functions in embryo culture, such as a chelator of heavy metals, free radical scavenger, pH and osmotic regulator, a stabilizer, growth factor carrier, a surfactant, and a nutrient. However, the commercially available human serum albumin (HSA) products may not be completely safe since they could be contaminated with viruses and prions. Recombinant human serum albumin (rHSA) has been reported to be as efficient as commercial HSA for fertilization and embryo development. Despite the possible benefits of rHSA, it has not been widely used for embryo culture due to its high cost of production. Our objective was to analyze the redox state of different types of HSA products and rHSA to define oxidative status batch variations of HSA and rHSA and to evaluate the optimal concentration of rHSA for mouse embryo culture. The redox state of the HSA and rHSA used in embryo culture media was found to vary widely. Redox variations were found among different HSA batches as well as among rHSA batches. The highest blastocyst development and hatching rates were obtained with rHSA used at a concentration of 0.05mg/mL. We showed that very low concentrations of rHSA were most favorable for advanced mouse embryo development in culture.
AB - Albumin has multiple physiological functions in embryo culture, such as a chelator of heavy metals, free radical scavenger, pH and osmotic regulator, a stabilizer, growth factor carrier, a surfactant, and a nutrient. However, the commercially available human serum albumin (HSA) products may not be completely safe since they could be contaminated with viruses and prions. Recombinant human serum albumin (rHSA) has been reported to be as efficient as commercial HSA for fertilization and embryo development. Despite the possible benefits of rHSA, it has not been widely used for embryo culture due to its high cost of production. Our objective was to analyze the redox state of different types of HSA products and rHSA to define oxidative status batch variations of HSA and rHSA and to evaluate the optimal concentration of rHSA for mouse embryo culture. The redox state of the HSA and rHSA used in embryo culture media was found to vary widely. Redox variations were found among different HSA batches as well as among rHSA batches. The highest blastocyst development and hatching rates were obtained with rHSA used at a concentration of 0.05mg/mL. We showed that very low concentrations of rHSA were most favorable for advanced mouse embryo development in culture.
KW - Embryo culture
KW - Human serum albumin product
KW - Recombinant human serum albumin
KW - Redox state
UR - http://www.scopus.com/inward/record.url?scp=84872533233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872533233&partnerID=8YFLogxK
U2 - 10.3109/19396368.2012.727946
DO - 10.3109/19396368.2012.727946
M3 - Article
C2 - 23050831
AN - SCOPUS:84872533233
SN - 1939-6368
VL - 59
SP - 48
EP - 52
JO - Systems Biology in Reproductive Medicine
JF - Systems Biology in Reproductive Medicine
IS - 1
ER -
