The regulation of sulfur use by ferrous ion in thiobacillus ferrooxidans

Tsuyoshi Sugio, Toru Hirose, Aya Oto, Kenji Inagaki, Tatsuo Tano

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10 Citations (Scopus)


The use of elemental sulfur (S°) as an energy source by T. ferrooxidans AP19-3 was completely inhibited by above 108mM of Fe2+ added to S° (l%)-salts medium, in which about 0.6 mM Fe2+remained after 30 days cultivation without being oxidized. In contrast, the use of Fe2+ by this strain was not inhibited by S°. It was found that specific activities of hydrogen sulfide: ferric ion oxidoreductase (SFORase) and sulfite: ferric ion oxidoreductase, which are crucial in sulfur and sulfite oxidations of this strain, were completely inhibited by 20 mM and 1 mM Fe2+, suggesting that this may be a main cause for the cells not using sulfur as an energy source. The SFORase activity of the cells incubated with S°-Fe2+-salts medium decreased to 39% of that in control cells without Fe2+, suggesting that Fe2+ added to the medium inhibits a sulfite: ferric ion oxidoreductase, and as a results sulfite ions are accumulated in the cells and this damages the SFORase. Sodium sulfite completely inhibited cell growth on S°-salts medium at 0.1 m M, but not on iron-salts medium.

Original languageEnglish
Pages (from-to)2017-2022
Number of pages6
JournalAgricultural and Biological Chemistry
Issue number8
Publication statusPublished - Aug 1990

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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