The role of bone marrow-derived cells during the bone healing process in the GFP mouse bone marrow transplantation model

Hidetsugu Tsujigiwa, Yasuhisa Hirata, Naoki Katase, Rosario Rivera Buery, Ryo Tamamura, Satoshi Ito, Shin Takagi, Seiji Iida, Hitoshi Nagatsuka

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Bone healing is a complex and multistep process in which the origin of the cells participating in bone repair is still unknown. The involvement of bone marrow-derived cells in tissue repair has been the subject of recent studies. In the present study, bone marrow-derived cells in bone healing were traced using the GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) were transplanted into C57BL/6 J wild mice. After transplantation, bone injury was created using a 1.0-mm drill. Bone healing was histologically assessed at 3, 7, 14, and 28 postoperative days. Immunohistochemistry for GFP; double-fluorescent immunohistochemistry for GFP-F4/80, GFP-CD34, and GFP-osteocalcin; and double-staining for GFP and tartrate-resistant acid phosphatase were performed. Bone marrow transplantation successfully replaced the hematopoietic cells into GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts or osteocytes in the repair stage were GFP-negative, whereas osteoclasts in the repair and remodeling stages and hematopoietic cells were GFP-positive. The results indicated that bone marrow-derived cells might not differentiate into osteoblasts. The role of bone marrow-derived cells might be limited to adjustment of the microenvironment by differentiating into inflammatory cells, osteoclasts, or endothelial cells in immature blood vessels.

Original languageEnglish
Pages (from-to)296-306
Number of pages11
JournalCalcified Tissue International
Issue number3
Publication statusPublished - Mar 2013


  • Bone fracture
  • Bone healing
  • Bone marrow transplantation
  • Bone marrow-derived cell
  • GFP

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology


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