The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen: contamination by extraencephalic mast-cell histamine

Ryozo Oishi; Masahiro Nishibori; Kiyomi Saeki

doi:10.1016/0006-8993(83)91302-1

The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen: contamination by extraencephalic mast-cell histamine

Ryozo Oishi, Masahiro Nishibori, Kiyomi Saeki

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.

Original language	English
Pages (from-to)	180-183
Number of pages	4
Journal	Brain Research
Volume	261
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)91302-1
Publication status	Published - Feb 14 1983

Keywords

HPLC
brain
compound 48/80
histamine
liquid nitrogen freezing
microwave irradiation
tele-methylhistamine

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(83)91302-1

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The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen: contamination by extraencephalic mast-cell histamine. / Oishi, Ryozo; Nishibori, Masahiro; Saeki, Kiyomi.
In: Brain Research, Vol. 261, No. 1, 14.02.1983, p. 180-183.

Research output: Contribution to journal › Article › peer-review

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The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen: contamination by extraencephalic mast-cell histamine

Abstract

Keywords

ASJC Scopus subject areas

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