The specific localization of advanced glycation end-products (AGEs) in rat pancreatic islets

Yuta Morioka, Kiyoshi Teshigawara, Yasuko Tomono, Dengli Wang, Yasuhisa Izushi, Hidenori Wake, Keyue Liu, Hideo Kohka Takahashi, Shuji Mori, Masahiro Nishibori

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Advanced glycation end-products (AGEs) are produced by non-enzymatic glycation between protein and reducing sugar such as glucose. Although glyceraldehyde-derived AGEs (Glycer-AGEs), one of the AGEs subspecies, have been reported to be involved in the pathogenesis of various age-relating diseases such as diabetes mellitus or arteriosclerosis, little is known about the pathological and physiological mechanism of AGEs in vivo. In present study, we produced 4 kinds of polyclonal antibodies against AGEs subspecies and investigated the localization of AGEs-modified proteins in rat peripheral tissues, making use of these antibodies. We found that Glycer-AGEs and methylglyoxal-derived AGEs (MGO-AGEs) were present in pancreatic islets of healthy rats, distinguished clearly into the pancreatic α and β cells, respectively. Although streptozotocin-induced diabetic rats suffered from remarkable impairment of pancreatic islets, the localization and deposit levels of the Glycer- and MGO-AGEs were not altered in the remaining α and β cells. Remarkably, the MGO-AGEs in pancreatic β cells were localized into the insulin-secretory granules. These results suggest that the cell-specific localization of AGEs-modified proteins are presence generally in healthy peripheral tissues, involved in physiological intracellular roles, such as a post-translational modulator contributing to the secretory and/or maturational functions of insulin.

Original languageEnglish
Pages (from-to)218-224
Number of pages7
JournalJournal of Pharmacological Sciences
Issue number4
Publication statusPublished - Aug 2017


  • Advanced glycation end products (AGEs)
  • Glyceraldehyde-derived AGEs (Glycer-AGEs)
  • Methylglyoxal-derived AGEs (MGO-AGEs)
  • Pancreatic alpha cells
  • Pancreatic beta cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology


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