TY - JOUR
T1 - The thylakoid carbonic anhydrase associated with photosystem II is the component of inorganic carbon accumulating system in cells of halo- and alkaliphilic cyanobacterium Rhabdoderma lineare
AU - Dudoladova, Marina V.
AU - Kupriyanova, Elena V.
AU - Markelova, Alexandra G.
AU - Sinetova, Maria P.
AU - Allakhverdiev, Suleyman I.
AU - Pronina, Natalia A.
N1 - Funding Information:
We are very grateful to Professor G. Samuelsson (Umea University, Sweden) who kindly supplied us with primary antibodies. We thank Professor G.A. Zavarzin and Dr. L.M. Gerasimenko (Institute of Microbiology RAS, Moscow) who provided us with the strain of R. lineare. This work was supported by grants to N.A.P. and S.I.A from the Russian Foundation for Basic Research (nos. 04-04-49407 and 05-04-49672), by grant from the Ministry of Science and Education (MK-3393.2005.4) to E.V.K., and by grants from “Biosphere Evolution” and “Molecular and Cell Biology” programs from Russian Academy of Sciences.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/6
Y1 - 2007/6
N2 - The organization of carbonic anhydrase (CA) system in halo- and alkaliphilic cyanobacterium Rhabdoderma lineare was studied by Western blot analysis and immunocytochemical electron microscopy. The presence of putative extracellular α-CA of 60 kDa in the glycocalyx, forming a tight sheath around the cell, and of two intracellular β-CA is reported. We show for the first time that the β-CA of 60 kDa is expressed constitutively and associated with polypeptides of photosystem II (β-CA-PS II). Another soluble β-CA of 25 kDa was induced in low-bicarbonate medium. Induction of synthesis of the latter β-CA was accompanied by an increase in the intracellular pool of inorganic carbon, which suggests an important role of this enzyme in the functioning of a CO2-concentrating mechanism.
AB - The organization of carbonic anhydrase (CA) system in halo- and alkaliphilic cyanobacterium Rhabdoderma lineare was studied by Western blot analysis and immunocytochemical electron microscopy. The presence of putative extracellular α-CA of 60 kDa in the glycocalyx, forming a tight sheath around the cell, and of two intracellular β-CA is reported. We show for the first time that the β-CA of 60 kDa is expressed constitutively and associated with polypeptides of photosystem II (β-CA-PS II). Another soluble β-CA of 25 kDa was induced in low-bicarbonate medium. Induction of synthesis of the latter β-CA was accompanied by an increase in the intracellular pool of inorganic carbon, which suggests an important role of this enzyme in the functioning of a CO2-concentrating mechanism.
KW - Alkaliphilic cyanobacteria
KW - Carbonic anhydrase immunolocalization
KW - Intracellular pool of C
KW - Photosystem II
KW - Rhabdoderma lineare
KW - Soda lakes
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U2 - 10.1016/j.bbabio.2006.12.006
DO - 10.1016/j.bbabio.2006.12.006
M3 - Article
C2 - 17292848
AN - SCOPUS:34249776071
SN - 0005-2728
VL - 1767
SP - 616
EP - 623
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 6
ER -