TY - CHAP
T1 - The use of VIGS technology to study plant-herbivore interactions
AU - Galis, Ivan
AU - Schuman, Meredith C.
AU - Gase, Klaus
AU - Hettenhausen, Christian
AU - Hartl, Markus
AU - Dinh, Son T.
AU - Wu, Jianqiang
AU - Bonaventure, Gustavo
AU - Baldwin, Ian T.
PY - 2013
Y1 - 2013
N2 - Plants employ a large variety of defense strategies to resist herbivores, which require transcriptional reprogramming of cells and profound changes in plant metabolism. Due to the large number of genes involved in defense processes, rapid screening strategies are essential for elucidating the contributions of individual genes in the responses of plants to herbivory. However, databases and seed banks of mutant plants which allow rapid retrieval of mutant genotypes are limited to a few model plant species, namely, Arabidopsis thaliana and Oryza sativa (rice). In other plants, virus-induced gene silencing (VIGS) offers an efficient alternative for screening the functions of individual genes in order to prioritize the allocations of the large time investments required to establish stably transformed RNAi-silenced lines. With VIGS, it is usually possible to achieve strong, specific silencing of target genes in the ecological models Nicotiana attenuata and Solanum nigrum , allowing the rapid assessment of gene silencing effects on phytohormone accumulation, signal transduction and accumulation of defense metabolites. VIGS plants are also useful in bioassays with specialist and generalist herbivores, allowing direct verification of gene function in plant resistance to herbivores.
AB - Plants employ a large variety of defense strategies to resist herbivores, which require transcriptional reprogramming of cells and profound changes in plant metabolism. Due to the large number of genes involved in defense processes, rapid screening strategies are essential for elucidating the contributions of individual genes in the responses of plants to herbivory. However, databases and seed banks of mutant plants which allow rapid retrieval of mutant genotypes are limited to a few model plant species, namely, Arabidopsis thaliana and Oryza sativa (rice). In other plants, virus-induced gene silencing (VIGS) offers an efficient alternative for screening the functions of individual genes in order to prioritize the allocations of the large time investments required to establish stably transformed RNAi-silenced lines. With VIGS, it is usually possible to achieve strong, specific silencing of target genes in the ecological models Nicotiana attenuata and Solanum nigrum , allowing the rapid assessment of gene silencing effects on phytohormone accumulation, signal transduction and accumulation of defense metabolites. VIGS plants are also useful in bioassays with specialist and generalist herbivores, allowing direct verification of gene function in plant resistance to herbivores.
KW - Herbivory
KW - Nicotiana attenuata
KW - Plant hormone level measurements
KW - Post-transcriptional gene silencing (PTGS)
KW - Secondary metabolite profiling
KW - Solanum nigrum
UR - http://www.scopus.com/inward/record.url?scp=84880154856&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880154856&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-278-0_9
DO - 10.1007/978-1-62703-278-0_9
M3 - Chapter
C2 - 23386299
AN - SCOPUS:84880154856
SN - 9781627032773
T3 - Methods in Molecular Biology
SP - 109
EP - 137
BT - Virus-Induced Gene Silencing
PB - Humana Press Inc.
ER -