Thermal stability of immobilized lipase from candida antarctica in glycerols with various water contents at elevated temperatures

Takashi Kobayashi; Takemasa Matsuo; Yukitaka Kimura; Shuji Adachi

doi:10.1007/s11746-008-1285-z

Thermal stability of immobilized lipase from candida antarctica in glycerols with various water contents at elevated temperatures

Takashi Kobayashi, Takemasa Matsuo, Yukitaka Kimura, Shuji Adachi

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80-100 °C to measure the residual activity as a function of time. The glycerol-containing water stabilized the immobilized CALB, especially at 30-60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.

Original language	English
Pages (from-to)	1041-1044
Number of pages	4
Journal	JAOCS, Journal of the American Oil Chemists' Society
Volume	85
Issue number	11
DOIs	https://doi.org/10.1007/s11746-008-1285-z
Publication status	Published - Nov 2008
Externally published	Yes

Keywords

Candida antarctica fraction B lipase
Gaussian distribution
Glycerol
Immobilization
Thermal inactivation

ASJC Scopus subject areas

Chemical Engineering(all)
Organic Chemistry

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10.1007/s11746-008-1285-z

Cite this

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title = "Thermal stability of immobilized lipase from candida antarctica in glycerols with various water contents at elevated temperatures",

abstract = "The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80-100 °C to measure the residual activity as a function of time. The glycerol-containing water stabilized the immobilized CALB, especially at 30-60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.",

keywords = "Candida antarctica fraction B lipase, Gaussian distribution, Glycerol, Immobilization, Thermal inactivation",

author = "Takashi Kobayashi and Takemasa Matsuo and Yukitaka Kimura and Shuji Adachi",

note = "Funding Information: Acknowledgments This study was supported by a Center-of-Excellence (COE) project for Microbial-Process Development Pioneering Future Production Systems, and was also supported as a Food Nanotechnology Project by the Ministry of Agriculture, Forestry, and Fisheries, Japan.",

year = "2008",

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doi = "10.1007/s11746-008-1285-z",

language = "English",

volume = "85",

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T1 - Thermal stability of immobilized lipase from candida antarctica in glycerols with various water contents at elevated temperatures

AU - Kobayashi, Takashi

AU - Matsuo, Takemasa

AU - Kimura, Yukitaka

AU - Adachi, Shuji

N1 - Funding Information: Acknowledgments This study was supported by a Center-of-Excellence (COE) project for Microbial-Process Development Pioneering Future Production Systems, and was also supported as a Food Nanotechnology Project by the Ministry of Agriculture, Forestry, and Fisheries, Japan.

PY - 2008/11

Y1 - 2008/11

N2 - The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80-100 °C to measure the residual activity as a function of time. The glycerol-containing water stabilized the immobilized CALB, especially at 30-60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.

AB - The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80-100 °C to measure the residual activity as a function of time. The glycerol-containing water stabilized the immobilized CALB, especially at 30-60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.

KW - Candida antarctica fraction B lipase

KW - Gaussian distribution

KW - Glycerol

KW - Immobilization

KW - Thermal inactivation

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JO - Oil & Soap

JF - Oil & Soap

IS - 11

ER -

Thermal stability of immobilized lipase from candida antarctica in glycerols with various water contents at elevated temperatures

Abstract

Keywords

ASJC Scopus subject areas

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