Thioredoxin deficiency increases oxidative stress and causes bilateral symmetrical degeneration in rat midbrain

Iori Ohmori, Mamoru Ouchida, Hirohiko Imai, Saeko Ishida, Shinya Toyokuni, Tomoji Mashimo

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Thioredoxin, encoded by Txn1, acts as a critical antioxidant in the defense against oxidative stress by regulating the dithiol/disulfide balance of interacting proteins. The role of thioredoxin in the central nervous system (CNS) is largely unknown. A phenotype-driven study of N-ethyl-N-nitrosourea-mutated rats with wild-running seizures revealed the importance of Txn1 mutations in CNS degeneration. Genetic mapping identified Txn1-F54L in the epileptic rats. The insulin-reducing activity of Txn1-F54L was approximately one-third of that of the wild-type (WT). Bilateral symmetrical vacuolar degeneration in the midbrain, mainly in the thalamus and the inferior colliculus, was observed in the Txn1-F54L rats. The lesions displayed neuronal and oligodendrocytic cell death. Neurons in Txn1-F54L rats showed morphological changes in the mitochondria. Vacuolar degeneration peaked at five weeks of age, and spontaneous repair began at seven weeks. The TUNEL assay showed that fibroblasts derived from homozygotes were susceptible to cell death under oxidative stress. In five-week-old WT rats, energy metabolism in the thalamus was significantly higher than that in the cerebral cortex. In conclusion, in juvenile rats, Txn1 seems to play an essential role in reducing oxidative stress in the midbrains with high energy metabolism.

Original languageEnglish
Article number105921
JournalNeurobiology of Disease
Publication statusPublished - Dec 2022


  • Epilepsy
  • Mitochondria
  • Oxidative stress
  • Thioredoxin
  • Txn1
  • Vacuolar degeneration

ASJC Scopus subject areas

  • Neurology


Dive into the research topics of 'Thioredoxin deficiency increases oxidative stress and causes bilateral symmetrical degeneration in rat midbrain'. Together they form a unique fingerprint.

Cite this