Abstract
In order to study the three-dimensional ultrastructure of the glomerular basement membrane (GBM), isolated GBM was treated with elastase, sodium dodecyl sulfate, and 2-mercaptoethanol and examined by transmission electron microscopy using ultrathin sectioning method and stereoscopic view. This treatment revealed clearly three-dimensional meshwork structure of GBM mainly composed of type IV collagen. Numerous regular polygonal small pores were present in the meshwork structure. The average short dimension of the small pores was 4.1±1.8nm and the average long dimension was 4.8±2.2 nm. Our present results further support the molecular sieve theory of the basement membrane.
| Original language | English |
|---|---|
| Pages (from-to) | 182-185 |
| Number of pages | 4 |
| Journal | Journal of Electron Microscopy |
| Volume | 39 |
| Issue number | 3 |
| Publication status | Published - Jun 1 1990 |
Keywords
- Chemical treatment
- Glomerular basement membrane
- Three-dimensional structure
- Type IV collagen
- Ultrastructure
ASJC Scopus subject areas
- Instrumentation