TY - JOUR
T1 - Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae
AU - Ohkita, Shuhei
AU - Lee, Yui
AU - Nguyen, Quyet
AU - Ikeda, Kenichi
AU - Suzuki, Nobuhiro
AU - Nakayashiki, Hitoshi
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (A) and (B) from the Japan Society for the Promotion of Science ( #25252011 and #25292028 ).
Publisher Copyright:
© 2019
PY - 2019/8
Y1 - 2019/8
N2 - Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.
AB - Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.
KW - Mycovirus
KW - Ourmia-like virus
KW - Subviral RNA
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U2 - 10.1016/j.virol.2019.05.015
DO - 10.1016/j.virol.2019.05.015
M3 - Article
C2 - 31170545
AN - SCOPUS:85067056677
SN - 0042-6822
VL - 534
SP - 25
EP - 35
JO - Virology
JF - Virology
ER -