TY - JOUR
T1 - Time-course effects of a single administration of cocaine on receptor binding and subunit mRNAs of GABA(A) receptors
AU - Yamaguchi, Mika
AU - Suzuki, Toshihito
AU - Abe, Shuzo
AU - Baba, Atsuomi
AU - Ito, Takehiko
AU - Okado, Nobuo
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research (C) 08671072 from the Ministry of Education, Science and Culture to TS.
PY - 2000/9/30
Y1 - 2000/9/30
N2 - We investigated the time-course effects of a single administration of cocaine (20 mg/kg) on GABA(A) receptor binding labeled by t- [35S]butylbicyclophophorothionate (TBPS) and on several types of GABA(A) receptor subunit mRNAs in the rat brain by in vitro quantitative receptor autoradiography and in situ hybridization. The levels of α1, β2, and β3 subunit mRNAs in several brain regions such as the cortex, cerebellum, and striatum were significantly decreased within 1 h, while β3 subunit mRNA was increased in the dentate gyrus. All of these changes were transient, occurring within 1 h after the injection of cocaine. In the cortex and cerebellum, the reduction in α1 subunit mRNA was followed by a significant decrease in [35S]TBPS receptor binding, which occurred 4 h after cocaine injection. These findings suggest that acute cocaine administration discretely regulates GABA(A) receptor subunit mRNA levels in several brain regions through a change in transcription or turnover rates of subunit mRNAs, which may be closely related to cocaine-induced behavioral abnormalities. (C) 2000 Elsevier Science B.V.
AB - We investigated the time-course effects of a single administration of cocaine (20 mg/kg) on GABA(A) receptor binding labeled by t- [35S]butylbicyclophophorothionate (TBPS) and on several types of GABA(A) receptor subunit mRNAs in the rat brain by in vitro quantitative receptor autoradiography and in situ hybridization. The levels of α1, β2, and β3 subunit mRNAs in several brain regions such as the cortex, cerebellum, and striatum were significantly decreased within 1 h, while β3 subunit mRNA was increased in the dentate gyrus. All of these changes were transient, occurring within 1 h after the injection of cocaine. In the cortex and cerebellum, the reduction in α1 subunit mRNA was followed by a significant decrease in [35S]TBPS receptor binding, which occurred 4 h after cocaine injection. These findings suggest that acute cocaine administration discretely regulates GABA(A) receptor subunit mRNA levels in several brain regions through a change in transcription or turnover rates of subunit mRNAs, which may be closely related to cocaine-induced behavioral abnormalities. (C) 2000 Elsevier Science B.V.
KW - Cocaine
KW - GABA(A) receptor
KW - Subunit mRNA
KW - Time course
KW - [S]TBPS binding
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U2 - 10.1016/S0169-328X(00)00166-2
DO - 10.1016/S0169-328X(00)00166-2
M3 - Article
C2 - 11000487
AN - SCOPUS:0034734839
SN - 0006-8993
VL - 81
SP - 155
EP - 163
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -