Tissue-specific mRNA expression profiles of drug-metabolizing enzymes and transporters in the cynomolgus monkey

Masuhiro Nishimura; Akiko Koeda; Hiroshi Morikawa; Tetsuo Satoh; Shizuo Narimatsu; Shinsaku Naito

doi:10.2133/dmpk.24.139

Tissue-specific mRNA expression profiles of drug-metabolizing enzymes and transporters in the cynomolgus monkey

Masuhiro Nishimura, Akiko Koeda, Hiroshi Morikawa, Tetsuo Satoh, Shizuo Narimatsu, Shinsaku Naito

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Pairs of forward and reverse primers and TaqMan probes specific to each of 19 drug-metabolizing enzymes (cytochrome P450s, UDP-glucuronosyltransferases, glutathione S-transferases, and sulfotransferases) and 5 transporters (ABC and SLC transporters) in the cynomolgus monkey were prepared. The expression level of each target mRNA was analyzed in total RNA obtained from three specimens of various cynomolgus monkey tissues (adrenal gland, brain, heart, kidney, large intestine, liver, lung, pancreas, prostate, small intestine, spleen, testis, and thymus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. The data obtained in the present study provide useful information on tissue-specific profiles of the expression of these target mRNAs in the cynomolgus monkey, and the results are expected to be valuable in establishing drug metabolism- and transporter-mediated screening systems using the cynomolgus monkey for the evaluation of new chemical entities in new drug development.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Drug Metabolism And Pharmacokinetics
Volume	24
Issue number	2
DOIs	https://doi.org/10.2133/dmpk.24.139
Publication status	Published - 2009
Externally published	Yes

Keywords

Cynomolgus monkey
Drug-metabolizing enzymes
MRNA expression
Quantification
Tissue distribution

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science
Pharmacology (medical)

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10.2133/dmpk.24.139

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title = "Tissue-specific mRNA expression profiles of drug-metabolizing enzymes and transporters in the cynomolgus monkey",

abstract = "Pairs of forward and reverse primers and TaqMan probes specific to each of 19 drug-metabolizing enzymes (cytochrome P450s, UDP-glucuronosyltransferases, glutathione S-transferases, and sulfotransferases) and 5 transporters (ABC and SLC transporters) in the cynomolgus monkey were prepared. The expression level of each target mRNA was analyzed in total RNA obtained from three specimens of various cynomolgus monkey tissues (adrenal gland, brain, heart, kidney, large intestine, liver, lung, pancreas, prostate, small intestine, spleen, testis, and thymus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. The data obtained in the present study provide useful information on tissue-specific profiles of the expression of these target mRNAs in the cynomolgus monkey, and the results are expected to be valuable in establishing drug metabolism- and transporter-mediated screening systems using the cynomolgus monkey for the evaluation of new chemical entities in new drug development.",

keywords = "Cynomolgus monkey, Drug-metabolizing enzymes, MRNA expression, Quantification, Tissue distribution",

author = "Masuhiro Nishimura and Akiko Koeda and Hiroshi Morikawa and Tetsuo Satoh and Shizuo Narimatsu and Shinsaku Naito",

year = "2009",

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pages = "139--144",

journal = "Drug Metabolism and Pharmacokinetics",

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AU - Nishimura, Masuhiro

AU - Koeda, Akiko

AU - Morikawa, Hiroshi

AU - Satoh, Tetsuo

AU - Narimatsu, Shizuo

AU - Naito, Shinsaku

PY - 2009

Y1 - 2009

N2 - Pairs of forward and reverse primers and TaqMan probes specific to each of 19 drug-metabolizing enzymes (cytochrome P450s, UDP-glucuronosyltransferases, glutathione S-transferases, and sulfotransferases) and 5 transporters (ABC and SLC transporters) in the cynomolgus monkey were prepared. The expression level of each target mRNA was analyzed in total RNA obtained from three specimens of various cynomolgus monkey tissues (adrenal gland, brain, heart, kidney, large intestine, liver, lung, pancreas, prostate, small intestine, spleen, testis, and thymus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. The data obtained in the present study provide useful information on tissue-specific profiles of the expression of these target mRNAs in the cynomolgus monkey, and the results are expected to be valuable in establishing drug metabolism- and transporter-mediated screening systems using the cynomolgus monkey for the evaluation of new chemical entities in new drug development.

AB - Pairs of forward and reverse primers and TaqMan probes specific to each of 19 drug-metabolizing enzymes (cytochrome P450s, UDP-glucuronosyltransferases, glutathione S-transferases, and sulfotransferases) and 5 transporters (ABC and SLC transporters) in the cynomolgus monkey were prepared. The expression level of each target mRNA was analyzed in total RNA obtained from three specimens of various cynomolgus monkey tissues (adrenal gland, brain, heart, kidney, large intestine, liver, lung, pancreas, prostate, small intestine, spleen, testis, and thymus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. The data obtained in the present study provide useful information on tissue-specific profiles of the expression of these target mRNAs in the cynomolgus monkey, and the results are expected to be valuable in establishing drug metabolism- and transporter-mediated screening systems using the cynomolgus monkey for the evaluation of new chemical entities in new drug development.

KW - Cynomolgus monkey

KW - Drug-metabolizing enzymes

KW - MRNA expression

KW - Quantification

KW - Tissue distribution

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Tissue-specific mRNA expression profiles of drug-metabolizing enzymes and transporters in the cynomolgus monkey

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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