Titanium implant functionalization with phosphate-containing polymers may favour in vivo osseointegration

Marcio V. Cardoso, Judith de Rycker, Amol Chaudhari, Eduardo Coutinho, Yasuhiro Yoshida, Bart Van Meerbeek, Marcelo F. Mesquita, Wander J. da Silva, Kumiko Yoshihara, Katleen Vandamme, Joke Duyck

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Aim: Osseointegration of titanium implants is predictable, but can be improved via surface functionalization. Materials and Methods: One hundred and twenty implants were installed in parietal bone of 12 domestic pigs and left to heal for 1 or 3 months. Five groups were defined according surface treatments: immersion in water (H2O), 10% polyphosphoric acid (PPA10), 1% phosphorylated pullulan (PPL1), 10% phosphorylated pullulan (PPL10) or 10% phosphorylated pullulan + 1 μg bone morphogenetic protein-2 (PPL10 BMP). As primary outcome, implant osseointegration was evaluated by quantitative histology, namely peri-implant bone formation (B/T in %) and bone-to-implant contact (BIC in %) for each healing period. The Wilcoxon signed-rank test and Mann–Whitney U-test with α = 0.05 were performed. Results: PPL10 and PPA10 groups showed significantly higher B/T and BIC results than the control (H2O) group at 1-month (p <.05). No significant difference was found between PPL1 and H2O or between PPL10 BMP and H2O, irrespective of healing time (1 or 3 months) or investigated parameter (B/T and BIC; p >.05). After 3 months, no experimental group showed a significant difference compared to the control group (H2O) for both investigated parameters (B/T and BIC; p >.05). Conclusion: Functionalizing titanium implants with inorganic or organic phosphate-containing polymers at 10 wt% concentration may stimulate peri-implant bone formation and implant osseointegration at early healing times.

Original languageEnglish
Pages (from-to)950-960
Number of pages11
JournalJournal of Clinical Periodontology
Issue number9
Publication statusPublished - Sept 2017


  • functionalization
  • implant
  • osseointegration
  • pullulan
  • surface

ASJC Scopus subject areas

  • Periodontics


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