TRAIL expression up-regulated by interferon-γ via phosphorylation of STAT1 induces myeloma cell death

Yoshie Miura; Takayuki Tsujioka; Yasumitsu Nishimura; Haruko Sakaguchi; Megumi Maeda; Hiroaki Hayashi; Maolong Dong; Fuminori Hyodoh; Ken Ichiro Yata; Hideho Wada; Takashi Sugihara; Takemi Otsuki

TRAIL expression up-regulated by interferon-γ via phosphorylation of STAT1 induces myeloma cell death

Yoshie Miura, Takayuki Tsujioka, Yasumitsu Nishimura, Haruko Sakaguchi, Megumi Maeda, Hiroaki Hayashi, Maolong Dong, Fuminori Hyodoh, Ken Ichiro Yata, Hideho Wada, Takashi Sugihara, Takemi Otsuki

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Background: Although the cellular and molecular biological effects of interferon (IFN)-α have been well-investigated, the effects of IFN-γ are less understood. Materials and Methods: Eleven human myeloma cell lines with various myeloma-specific chromosomal translocations and overexpression of oncogenes were cultured with 1,000 U/ml of IFN-γ. In the KMS-20 cells, which showed growth inhibition due to IFN-γ, trail expression, status of the Janus kinase (JAK)/STAT pathway were analyzed. Results: KMS-20 cells showed marked up-regulation of trail, activation of STAT1 and TRAIL hyperproduction induced by IFN-γ. Conclusion: The effects of IFN-γ on growth inhibition of KMS-20 cells were characterized by activation of the JAK/STAT signalling pathway, particularly STAT1 phosphorylation, enhanced secretion of TRAIL, and auto/paracrine usage of secreted TRAIL to induce apoptotic cell death. From these results, IFN-γ may be considered one of the drugs to be used in future multidrug chemotherapeutic regimens for myeloma patients.

Original language	English
Pages (from-to)	4115-4124
Number of pages	10
Journal	Anticancer research
Volume	26
Issue number	6 B
Publication status	Published - Nov 1 2006
Externally published	Yes

Keywords

IFN-γ
Myeloma
STAT1
TRAIL

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{b690f1e087d6493093de0e13a181b7a5,

title = "TRAIL expression up-regulated by interferon-γ via phosphorylation of STAT1 induces myeloma cell death",

abstract = "Background: Although the cellular and molecular biological effects of interferon (IFN)-α have been well-investigated, the effects of IFN-γ are less understood. Materials and Methods: Eleven human myeloma cell lines with various myeloma-specific chromosomal translocations and overexpression of oncogenes were cultured with 1,000 U/ml of IFN-γ. In the KMS-20 cells, which showed growth inhibition due to IFN-γ, trail expression, status of the Janus kinase (JAK)/STAT pathway were analyzed. Results: KMS-20 cells showed marked up-regulation of trail, activation of STAT1 and TRAIL hyperproduction induced by IFN-γ. Conclusion: The effects of IFN-γ on growth inhibition of KMS-20 cells were characterized by activation of the JAK/STAT signalling pathway, particularly STAT1 phosphorylation, enhanced secretion of TRAIL, and auto/paracrine usage of secreted TRAIL to induce apoptotic cell death. From these results, IFN-γ may be considered one of the drugs to be used in future multidrug chemotherapeutic regimens for myeloma patients.",

keywords = "IFN-γ, Myeloma, STAT1, TRAIL",

author = "Yoshie Miura and Takayuki Tsujioka and Yasumitsu Nishimura and Haruko Sakaguchi and Megumi Maeda and Hiroaki Hayashi and Maolong Dong and Fuminori Hyodoh and Yata, {Ken Ichiro} and Hideho Wada and Takashi Sugihara and Takemi Otsuki",

year = "2006",

month = nov,

day = "1",

language = "English",

volume = "26",

pages = "4115--4124",

journal = "Anticancer research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "6 B",

}

TY - JOUR

T1 - TRAIL expression up-regulated by interferon-γ via phosphorylation of STAT1 induces myeloma cell death

AU - Miura, Yoshie

AU - Tsujioka, Takayuki

AU - Nishimura, Yasumitsu

AU - Sakaguchi, Haruko

AU - Maeda, Megumi

AU - Hayashi, Hiroaki

AU - Dong, Maolong

AU - Hyodoh, Fuminori

AU - Yata, Ken Ichiro

AU - Wada, Hideho

AU - Sugihara, Takashi

AU - Otsuki, Takemi

PY - 2006/11/1

Y1 - 2006/11/1

N2 - Background: Although the cellular and molecular biological effects of interferon (IFN)-α have been well-investigated, the effects of IFN-γ are less understood. Materials and Methods: Eleven human myeloma cell lines with various myeloma-specific chromosomal translocations and overexpression of oncogenes were cultured with 1,000 U/ml of IFN-γ. In the KMS-20 cells, which showed growth inhibition due to IFN-γ, trail expression, status of the Janus kinase (JAK)/STAT pathway were analyzed. Results: KMS-20 cells showed marked up-regulation of trail, activation of STAT1 and TRAIL hyperproduction induced by IFN-γ. Conclusion: The effects of IFN-γ on growth inhibition of KMS-20 cells were characterized by activation of the JAK/STAT signalling pathway, particularly STAT1 phosphorylation, enhanced secretion of TRAIL, and auto/paracrine usage of secreted TRAIL to induce apoptotic cell death. From these results, IFN-γ may be considered one of the drugs to be used in future multidrug chemotherapeutic regimens for myeloma patients.

AB - Background: Although the cellular and molecular biological effects of interferon (IFN)-α have been well-investigated, the effects of IFN-γ are less understood. Materials and Methods: Eleven human myeloma cell lines with various myeloma-specific chromosomal translocations and overexpression of oncogenes were cultured with 1,000 U/ml of IFN-γ. In the KMS-20 cells, which showed growth inhibition due to IFN-γ, trail expression, status of the Janus kinase (JAK)/STAT pathway were analyzed. Results: KMS-20 cells showed marked up-regulation of trail, activation of STAT1 and TRAIL hyperproduction induced by IFN-γ. Conclusion: The effects of IFN-γ on growth inhibition of KMS-20 cells were characterized by activation of the JAK/STAT signalling pathway, particularly STAT1 phosphorylation, enhanced secretion of TRAIL, and auto/paracrine usage of secreted TRAIL to induce apoptotic cell death. From these results, IFN-γ may be considered one of the drugs to be used in future multidrug chemotherapeutic regimens for myeloma patients.

KW - IFN-γ

KW - Myeloma

KW - STAT1

KW - TRAIL

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M3 - Article

C2 - 17201122

AN - SCOPUS:33845945377

SN - 0250-7005

VL - 26

SP - 4115

EP - 4124

JO - Anticancer research

JF - Anticancer research

IS - 6 B

ER -

TRAIL expression up-regulated by interferon-γ via phosphorylation of STAT1 induces myeloma cell death

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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