TY - JOUR
T1 - Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature
AU - Ito, Yusuke
AU - Saisho, Daisuke
AU - Nakazono, Mikio
AU - Tsutsumi, Nobuhiro
AU - Hirai, Atsushi
N1 - Funding Information:
We thank the National Institute of Agrobiological Resources Rice Genome Research Program for providing EST clones C2339 and C11112. We also thank Drs. S. Kishitani and K. Toriyama (Tohoku University), Dr S. Sakajo (Niigata College of Pharmacy) and Dr. S. Akita (The University of Tokyo) for helpful discussions. This work was supported by grants from the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) and by grants-in-aid from the Ministry of Education, Science and Culture of Japan to A.H. Y.I. acknowledges the support of JSPS Research Fellowships for Young Scientists.
PY - 1997/12/12
Y1 - 1997/12/12
N2 - We identified two genes for alternative oxidase (AOX) from rice. One AOX gene (designated AOX1a) is located approx. 1.9 kb downstream of another AOX gene (designated AOX1b). Comparison of the genomic and cDNA sequences of the two AOX genes showed that the AOX1a gene is interrupted by three introns, as are AOX genes of other plants. On the other hand, two introns are inserted in the AOX1b gene. The predicted AOX1a and AOX1b precursor proteins consist of 332 and 335 amino acid residues, respectively. A genomic Southern hybridization analysis indicated that rice has several AOX genes other than the two tandem-arranged AOX genes. Steady-state mRNA levels of both of the genes for AOX1a and AOX1b were increased under low temperature (4°C). However, no difference in the pattern of induction of transcription between the genes for AOX1a and AOX1b was observed.
AB - We identified two genes for alternative oxidase (AOX) from rice. One AOX gene (designated AOX1a) is located approx. 1.9 kb downstream of another AOX gene (designated AOX1b). Comparison of the genomic and cDNA sequences of the two AOX genes showed that the AOX1a gene is interrupted by three introns, as are AOX genes of other plants. On the other hand, two introns are inserted in the AOX1b gene. The predicted AOX1a and AOX1b precursor proteins consist of 332 and 335 amino acid residues, respectively. A genomic Southern hybridization analysis indicated that rice has several AOX genes other than the two tandem-arranged AOX genes. Steady-state mRNA levels of both of the genes for AOX1a and AOX1b were increased under low temperature (4°C). However, no difference in the pattern of induction of transcription between the genes for AOX1a and AOX1b was observed.
KW - AOX
KW - Cyanide-resistant respiration
KW - Induction of transcription
KW - Multigene family
KW - Oryza sativa
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U2 - 10.1016/S0378-1119(97)00502-7
DO - 10.1016/S0378-1119(97)00502-7
M3 - Article
C2 - 9426242
AN - SCOPUS:0030773522
SN - 0378-1119
VL - 203
SP - 121
EP - 129
JO - Gene
JF - Gene
IS - 2
ER -