TY - JOUR
T1 - Transcription factor EB influences invasion and migration in oral squamous cell carcinomas
AU - Sakamoto, H.
AU - Yamashita, K.
AU - Okamoto, K.
AU - Kadowaki, T.
AU - Sakai, E.
AU - Umeda, M.
AU - Tsukuba, T.
N1 - Funding Information:
The authors appreciate Dr. Kazuhisa Nishishita for suggestive advices. The present study was supported by JSPS KAKENHI grant numbers 15H05298, 16K15790, 16K11863, and 16H00687.
Funding Information:
The present study was supported by JSPS KAKENHI grant numbers
Publisher Copyright:
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved
PY - 2018/7
Y1 - 2018/7
N2 - Objective: Transcription factor EB (TFEB) is a master regulator of lysosomal biogenesis and plays an important role in various cancers. However, the function of TFEB in oral squamous cell carcinomas has not been examined. The aim of this study was to elucidate the role of TFEB in oral squamous cell carcinomas. Materials and Methods: Expression levels of TFEB were examined in six different human oral squamous carcinoma cells: HSC2, HSC3, HSC4, SAS, OSC20, and SCC25. Knockdown of TFEB using small interfering RNA in HSC2 and HSC4 cells was performed. Cell morphology was observed by immunofluorescence microscopy. Cell proliferation, invasion, and adhesion were analyzed. Results: Expression levels of TFEB were high in HSC2, moderate in HSC4 and SCC25, and low in HSC3 and OSC20 cells. Knockdown of TFEB did not affect proliferation of HSC2 and HSC4 cells, but did induced enlargement of lysosomes and endosomes in HSC4 cells. TFEB silencing reduced invasion and migration of these HSC cell squamous carcinoma cells; however, increased cell adhesion was also observed. Conclusion: TFEB knockdown reduces invasion and migration of cancer cells, likely through lysosomal regulation. Taken together, TFEB influences cell invasion and migration of oral squamous cell carcinomas.
AB - Objective: Transcription factor EB (TFEB) is a master regulator of lysosomal biogenesis and plays an important role in various cancers. However, the function of TFEB in oral squamous cell carcinomas has not been examined. The aim of this study was to elucidate the role of TFEB in oral squamous cell carcinomas. Materials and Methods: Expression levels of TFEB were examined in six different human oral squamous carcinoma cells: HSC2, HSC3, HSC4, SAS, OSC20, and SCC25. Knockdown of TFEB using small interfering RNA in HSC2 and HSC4 cells was performed. Cell morphology was observed by immunofluorescence microscopy. Cell proliferation, invasion, and adhesion were analyzed. Results: Expression levels of TFEB were high in HSC2, moderate in HSC4 and SCC25, and low in HSC3 and OSC20 cells. Knockdown of TFEB did not affect proliferation of HSC2 and HSC4 cells, but did induced enlargement of lysosomes and endosomes in HSC4 cells. TFEB silencing reduced invasion and migration of these HSC cell squamous carcinoma cells; however, increased cell adhesion was also observed. Conclusion: TFEB knockdown reduces invasion and migration of cancer cells, likely through lysosomal regulation. Taken together, TFEB influences cell invasion and migration of oral squamous cell carcinomas.
KW - invasion
KW - oral squamous cell carcinoma
KW - transcription factor EB
UR - http://www.scopus.com/inward/record.url?scp=85045846661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045846661&partnerID=8YFLogxK
U2 - 10.1111/odi.12826
DO - 10.1111/odi.12826
M3 - Article
C2 - 29316035
AN - SCOPUS:85045846661
SN - 1354-523X
VL - 24
SP - 741
EP - 748
JO - Oral Diseases
JF - Oral Diseases
IS - 5
ER -
