Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles

Kogen Okita; Engkong Tan; Hina Satone; Shigeharu Kinoshita; Shuichi Asakawa; Daisuke Ojima; Hideki Yamazaki; Kazutaka Sakiyama; Tomohiro Takatani; Osamu Arakawa; Atsushi Hagiwara; Yoshitaka Sakakura

doi:10.1007/s12562-017-1079-z

Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles

Kogen Okita, Engkong Tan, Hina Satone, Shigeharu Kinoshita, Shuichi Asakawa, Daisuke Ojima, Hideki Yamazaki, Kazutaka Sakiyama, Tomohiro Takatani, Osamu Arakawa, Atsushi Hagiwara, Yoshitaka Sakakura

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1 Citation (Scopus)

Abstract

To reveal the sensing of tetrodotoxin (TTX) by tiger puffer Takifugu rubripes juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the T. rubripes genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of T. rubripes juveniles is controlled in the feeding center in the brain, that T. rubripes may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.

Original language	English
Pages (from-to)	401-412
Number of pages	12
Journal	Fisheries science
Volume	83
Issue number	3
DOIs	https://doi.org/10.1007/s12562-017-1079-z
Publication status	Published - May 1 2017
Externally published	Yes

Keywords

Central nervous system
Feeding center
RNA sequencing
Reward system
Takifugu rubripes
Tetrodotoxin (TTX)

ASJC Scopus subject areas

Aquatic Science

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10.1007/s12562-017-1079-z

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Okita, K., Tan, E., Satone, H., Kinoshita, S., Asakawa, S., Ojima, D., Yamazaki, H., Sakiyama, K., Takatani, T., Arakawa, O., Hagiwara, A., & Sakakura, Y. (2017). Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles. Fisheries science, 83(3), 401-412. https://doi.org/10.1007/s12562-017-1079-z

Okita, K, Tan, E, Satone, H, Kinoshita, S, Asakawa, S, Ojima, D, Yamazaki, H, Sakiyama, K, Takatani, T, Arakawa, O, Hagiwara, A & Sakakura, Y 2017, 'Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles', Fisheries science, vol. 83, no. 3, pp. 401-412. https://doi.org/10.1007/s12562-017-1079-z

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title = "Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles",

abstract = "To reveal the sensing of tetrodotoxin (TTX) by tiger puffer Takifugu rubripes juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the T. rubripes genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of T. rubripes juveniles is controlled in the feeding center in the brain, that T. rubripes may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.",

keywords = "Central nervous system, Feeding center, RNA sequencing, Reward system, Takifugu rubripes, Tetrodotoxin (TTX)",

author = "Kogen Okita and Engkong Tan and Hina Satone and Shigeharu Kinoshita and Shuichi Asakawa and Daisuke Ojima and Hideki Yamazaki and Kazutaka Sakiyama and Tomohiro Takatani and Osamu Arakawa and Atsushi Hagiwara and Yoshitaka Sakakura",

note = "Funding Information: This study was financially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) to Y. S. (15K07581, 24380109, 21580227, 19580209), T. T. (26450287), and a Grant-in-Aid for JSPS Research Fellows from JSPS to K. O. (14J00997). This study was partly supported by the Nagasaki University Major Research Project to Y. S., T. T. and O. A. Publisher Copyright: {\textcopyright} 2017, Japanese Society of Fisheries Science.",

year = "2017",

month = may,

day = "1",

doi = "10.1007/s12562-017-1079-z",

language = "English",

volume = "83",

pages = "401--412",

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T1 - Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles

AU - Okita, Kogen

AU - Tan, Engkong

AU - Satone, Hina

AU - Kinoshita, Shigeharu

AU - Asakawa, Shuichi

AU - Ojima, Daisuke

AU - Yamazaki, Hideki

AU - Sakiyama, Kazutaka

AU - Takatani, Tomohiro

AU - Arakawa, Osamu

AU - Hagiwara, Atsushi

AU - Sakakura, Yoshitaka

N1 - Funding Information: This study was financially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) to Y. S. (15K07581, 24380109, 21580227, 19580209), T. T. (26450287), and a Grant-in-Aid for JSPS Research Fellows from JSPS to K. O. (14J00997). This study was partly supported by the Nagasaki University Major Research Project to Y. S., T. T. and O. A. Publisher Copyright: © 2017, Japanese Society of Fisheries Science.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - To reveal the sensing of tetrodotoxin (TTX) by tiger puffer Takifugu rubripes juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the T. rubripes genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of T. rubripes juveniles is controlled in the feeding center in the brain, that T. rubripes may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.

AB - To reveal the sensing of tetrodotoxin (TTX) by tiger puffer Takifugu rubripes juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the T. rubripes genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of T. rubripes juveniles is controlled in the feeding center in the brain, that T. rubripes may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.

KW - Central nervous system

KW - Feeding center

KW - RNA sequencing

KW - Reward system

KW - Takifugu rubripes

KW - Tetrodotoxin (TTX)

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U2 - 10.1007/s12562-017-1079-z

DO - 10.1007/s12562-017-1079-z

M3 - Article

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SN - 0919-9268

VL - 83

SP - 401

EP - 412

JO - Fisheries Science

JF - Fisheries Science

IS - 3

ER -

Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles

Abstract

Keywords

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