Purpose. To elucidate factors influencing transdifferentiation of retinal pigment epithelial (RPE) cells in suspension culture. Methods. RPE eel Is were isolated as a sheet from 7.5-day-old chick embryos and cultured either as a sheet or dissociated eelIs after trypsin treatment in a non-adherent dish for suspension culture for one month. Basic fibroblast growth factor (bFGF) was added to the medium at varying concentrations of 0-200 ng/ml. Results. At the higher concentrations (10 or 200 ng/ml) of bFGF, a rounded-up RPE sheet grew to develop a nonpigmented lump with an onion-like structure, while dissociated RPE cells, although they reaggregated, did not form such structure. Histologically, the onion-like structure consisted of lens-fiber-like cells, positive for ant i-crystall in antibody and anti-bFGF-receptor antibody by immunohistochemistry. The formation of this lens-1ike structure did not occur at the concentration of bFGF, 1 ng/ml or lower. Conclusion. The transdifferentiation of RPE cells into lens in suspension culture was dependent upon the concentration of bFGF as well as proper three-dimensional interaction among each RPE cell.
|Investigative Ophthalmology and Visual Science
|Published - Dec 1 1997
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience