Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation

Kenji Inagaki; Tatsuo Tano; Hidehiko Tanaka; Noriaki Kishimoto

doi:10.1271/bbb.57.1770

Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation

Kenji Inagaki, Tatsuo Tano, Hidehiko Tanaka, Noriaki Kishimoto

School of Agriculture

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH1O1 (8.8kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAU1 and a pUC19 carrying a β-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 × 10³ transformants per μg of plasmid DNA at a field strength of 10kV/cm with a 7.0ms pulse.

Original language	English
Pages (from-to)	1770-1771
Number of pages	2
Journal	Bioscience, biotechnology, and biochemistry
Volume	57
Issue number	10
DOIs	https://doi.org/10.1271/bbb.57.1770
Publication status	Published - 1993

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation",

abstract = "We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH1O1 (8.8kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAU1 and a pUC19 carrying a β-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 × 103 transformants per μg of plasmid DNA at a field strength of 10kV/cm with a 7.0ms pulse.",

author = "Kenji Inagaki and Tatsuo Tano and Hidehiko Tanaka and Noriaki Kishimoto",

note = "Funding Information: Acknowledgments. This work was supported in part by the Agricultural Chemical Foundation and by a Grant-in-Aid for Scientific Research (No. 02660121) from the Ministry of Education, Science, and Culture of Japan to K.I.",

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doi = "10.1271/bbb.57.1770",

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T1 - Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation

AU - Inagaki, Kenji

AU - Tano, Tatsuo

AU - Tanaka, Hidehiko

AU - Kishimoto, Noriaki

N1 - Funding Information: Acknowledgments. This work was supported in part by the Agricultural Chemical Foundation and by a Grant-in-Aid for Scientific Research (No. 02660121) from the Ministry of Education, Science, and Culture of Japan to K.I.

PY - 1993

Y1 - 1993

N2 - We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH1O1 (8.8kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAU1 and a pUC19 carrying a β-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 × 103 transformants per μg of plasmid DNA at a field strength of 10kV/cm with a 7.0ms pulse.

AB - We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH1O1 (8.8kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAU1 and a pUC19 carrying a β-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 × 103 transformants per μg of plasmid DNA at a field strength of 10kV/cm with a 7.0ms pulse.

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Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation

Abstract

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