Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb

Kyoko Saito; Shouichiro Saito; Kazuyuki Taniguchi; Naoto Kobayashi; Takehiro Terashita; Tetsuya Shimokawa; Katsumi Mominoki; Kyojy Miyawaki; Jie Chen; Shuang Yan Gao; Chun Yu Li; Seiji Matsuda

doi:10.1016/j.neures.2004.07.001

Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb

Kyoko Saito, Shouichiro Saito, Kazuyuki Taniguchi, Naoto Kobayashi, Takehiro Terashita, Tetsuya Shimokawa, Katsumi Mominoki, Kyojy Miyawaki, Jie Chen, Shuang Yan Gao, Chun Yu Li, Seiji Matsuda

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In the developing central nervous system, apoptosis plays an important role in the normal organization of the neuronal circuit. The timing of neurogenesis, proliferation, and migration of the neurons in the developing olfactory bulb (OB) is well studied; however, the involvement of apoptosis in this process is not fully understood. In this study, we examined the changes in the distribution and the number of apoptotic cells in the rat OB during embryonic and postnatal periods, by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) staining. Although the number of TUNEL-positive cells was relatively small during the embryonic period, it gradually increased after birth, and peaked on postnatal day 20 with statistical significance, especially in the granule cell layer of the main OB. This transient increase of TUNEL-positive cells on postnatal day 20 may be involved in a critical event during maturation of the OB.

Original language	English
Pages (from-to)	219-225
Number of pages	7
Journal	Neuroscience Research
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/j.neures.2004.07.001
Publication status	Published - Oct 2004
Externally published	Yes

Keywords

Apoptosis
Development
Olfactory bulb
Rat
TUNEL staining

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neures.2004.07.001

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@article{7f3cd7d6fbc94db786435e0c043ea79b,

title = "Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb",

abstract = "In the developing central nervous system, apoptosis plays an important role in the normal organization of the neuronal circuit. The timing of neurogenesis, proliferation, and migration of the neurons in the developing olfactory bulb (OB) is well studied; however, the involvement of apoptosis in this process is not fully understood. In this study, we examined the changes in the distribution and the number of apoptotic cells in the rat OB during embryonic and postnatal periods, by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) staining. Although the number of TUNEL-positive cells was relatively small during the embryonic period, it gradually increased after birth, and peaked on postnatal day 20 with statistical significance, especially in the granule cell layer of the main OB. This transient increase of TUNEL-positive cells on postnatal day 20 may be involved in a critical event during maturation of the OB.",

keywords = "Apoptosis, Development, Olfactory bulb, Rat, TUNEL staining",

author = "Kyoko Saito and Shouichiro Saito and Kazuyuki Taniguchi and Naoto Kobayashi and Takehiro Terashita and Tetsuya Shimokawa and Katsumi Mominoki and Kyojy Miyawaki and Jie Chen and Gao, {Shuang Yan} and Li, {Chun Yu} and Seiji Matsuda",

note = "Funding Information: We thank D. Shimizu in INCS for his technical instruction. This work was supported in part by a grant-in-aid (14370466 to S.M.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2004",

month = oct,

doi = "10.1016/j.neures.2004.07.001",

language = "English",

volume = "50",

pages = "219--225",

journal = "Neuroscience Research",

issn = "0168-0102",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb

AU - Saito, Kyoko

AU - Saito, Shouichiro

AU - Taniguchi, Kazuyuki

AU - Kobayashi, Naoto

AU - Terashita, Takehiro

AU - Shimokawa, Tetsuya

AU - Mominoki, Katsumi

AU - Miyawaki, Kyojy

AU - Chen, Jie

AU - Gao, Shuang Yan

AU - Li, Chun Yu

AU - Matsuda, Seiji

N1 - Funding Information: We thank D. Shimizu in INCS for his technical instruction. This work was supported in part by a grant-in-aid (14370466 to S.M.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2004/10

Y1 - 2004/10

N2 - In the developing central nervous system, apoptosis plays an important role in the normal organization of the neuronal circuit. The timing of neurogenesis, proliferation, and migration of the neurons in the developing olfactory bulb (OB) is well studied; however, the involvement of apoptosis in this process is not fully understood. In this study, we examined the changes in the distribution and the number of apoptotic cells in the rat OB during embryonic and postnatal periods, by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) staining. Although the number of TUNEL-positive cells was relatively small during the embryonic period, it gradually increased after birth, and peaked on postnatal day 20 with statistical significance, especially in the granule cell layer of the main OB. This transient increase of TUNEL-positive cells on postnatal day 20 may be involved in a critical event during maturation of the OB.

AB - In the developing central nervous system, apoptosis plays an important role in the normal organization of the neuronal circuit. The timing of neurogenesis, proliferation, and migration of the neurons in the developing olfactory bulb (OB) is well studied; however, the involvement of apoptosis in this process is not fully understood. In this study, we examined the changes in the distribution and the number of apoptotic cells in the rat OB during embryonic and postnatal periods, by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) staining. Although the number of TUNEL-positive cells was relatively small during the embryonic period, it gradually increased after birth, and peaked on postnatal day 20 with statistical significance, especially in the granule cell layer of the main OB. This transient increase of TUNEL-positive cells on postnatal day 20 may be involved in a critical event during maturation of the OB.

KW - Apoptosis

KW - Development

KW - Olfactory bulb

KW - Rat

KW - TUNEL staining

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Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb

Abstract

Keywords

ASJC Scopus subject areas

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