Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

Hiroshi Kuroda; Haruka Suzuki; Takahiro Kusumegi; Tetsuro Hirose; Yasushi Yukawa; Masahiro Sugiura

doi:10.1093/pcp/pcm097

Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

Hiroshi Kuroda, Haruka Suzuki, Takahiro Kusumegi, Tetsuro Hirose, Yasushi Yukawa, Masahiro Sugiura

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.

Original language	English
Pages (from-to)	1374-1378
Number of pages	5
Journal	Plant and Cell Physiology
Volume	48
Issue number	9
DOIs	https://doi.org/10.1093/pcp/pcm097
Publication status	Published - Sept 2007
Externally published	Yes

Keywords

GUG codon
In vitro
Shine-Dalgarno sequence
Tobacco
Translation
psbC

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

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10.1093/pcp/pcm097

Cite this

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title = "Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts",

abstract = "The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.",

keywords = "GUG codon, In vitro, Shine-Dalgarno sequence, Tobacco, Translation, psbC",

author = "Hiroshi Kuroda and Haruka Suzuki and Takahiro Kusumegi and Tetsuro Hirose and Yasushi Yukawa and Masahiro Sugiura",

note = "Funding Information: We thank Ms. Maki Yukawa for suggestions and discussions. This work was supported partly by the New Energy and Industrial Technology Development Organization (the Green Biotechnology Program), and by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (Nos. 09740595 and 10170214 to T.H. and No. 17370020 to M.S.).",

year = "2007",

month = sep,

doi = "10.1093/pcp/pcm097",

language = "English",

volume = "48",

pages = "1374--1378",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

publisher = "Oxford University Press",

number = "9",

}

TY - JOUR

T1 - Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

AU - Kuroda, Hiroshi

AU - Suzuki, Haruka

AU - Kusumegi, Takahiro

AU - Hirose, Tetsuro

AU - Yukawa, Yasushi

AU - Sugiura, Masahiro

N1 - Funding Information: We thank Ms. Maki Yukawa for suggestions and discussions. This work was supported partly by the New Energy and Industrial Technology Development Organization (the Green Biotechnology Program), and by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (Nos. 09740595 and 10170214 to T.H. and No. 17370020 to M.S.).

PY - 2007/9

Y1 - 2007/9

N2 - The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.

AB - The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.

KW - GUG codon

KW - In vitro

KW - Shine-Dalgarno sequence

KW - Tobacco

KW - Translation

KW - psbC

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DO - 10.1093/pcp/pcm097

M3 - Article

C2 - 17664183

AN - SCOPUS:34548808708

SN - 0032-0781

VL - 48

SP - 1374

EP - 1378

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 9

ER -

Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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