Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation

Rina Muraoka; Hidetsugu Tsujigiwa; Keisuke Nakano; Naoki Katase; Ryo Tamamura; Mihoko Tomida; Norimasa Okafuji; Hitoshi Nagatsuka; Toshiyuki Kawakami

doi:10.2485/jhtb.20.301

Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation

Rina Muraoka, Hidetsugu Tsujigiwa, Keisuke Nakano, Naoki Katase, Ryo Tamamura, Mihoko Tomida, Norimasa Okafuji, Hitoshi Nagatsuka, Toshiyuki Kawakami

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We examined the transplanted bone marrow-derived cell migration into periodontal tissues. Bone marrow cells from green fluorescent protein (GFP) transgenic mice were transplanted. The immunohistochemistry (IHC) revealed that GFP-positive cells were detected in the periodontal tissues. The GFP-positive cells histopathologically differentiated into some cell types. The fluorescence IHC and TRAP staining techniques demonstrated these cells were detected as osteoclasts and macrophages. Furthermore, GFP-positive cells gathered adjacent blood vessels. The data suggest that GFP-positive bone marrow-derived cell migrate into periodontal tissues and differentiate periodontal tissue component-cells.

Original language	English
Pages (from-to)	301-306
Number of pages	6
Journal	Journal of Hard Tissue Biology
Volume	20
Issue number	4
DOIs	https://doi.org/10.2485/jhtb.20.301
Publication status	Published - 2011

Keywords

Bone marrow-derived cells
Fibroblasts
Green fluorescent protein (GFP)
Macrophages
Osteoclasts
Periodontal tissues

ASJC Scopus subject areas

Medicine (miscellaneous)
Biochemistry
Biomaterials
Orthopedics and Sports Medicine
Dentistry(all)
Cell Biology

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10.2485/jhtb.20.301

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title = "Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation",

abstract = "We examined the transplanted bone marrow-derived cell migration into periodontal tissues. Bone marrow cells from green fluorescent protein (GFP) transgenic mice were transplanted. The immunohistochemistry (IHC) revealed that GFP-positive cells were detected in the periodontal tissues. The GFP-positive cells histopathologically differentiated into some cell types. The fluorescence IHC and TRAP staining techniques demonstrated these cells were detected as osteoclasts and macrophages. Furthermore, GFP-positive cells gathered adjacent blood vessels. The data suggest that GFP-positive bone marrow-derived cell migrate into periodontal tissues and differentiate periodontal tissue component-cells.",

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T1 - Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation

AU - Muraoka, Rina

AU - Tsujigiwa, Hidetsugu

AU - Nakano, Keisuke

AU - Katase, Naoki

AU - Tamamura, Ryo

AU - Tomida, Mihoko

AU - Okafuji, Norimasa

AU - Nagatsuka, Hitoshi

AU - Kawakami, Toshiyuki

PY - 2011

Y1 - 2011

N2 - We examined the transplanted bone marrow-derived cell migration into periodontal tissues. Bone marrow cells from green fluorescent protein (GFP) transgenic mice were transplanted. The immunohistochemistry (IHC) revealed that GFP-positive cells were detected in the periodontal tissues. The GFP-positive cells histopathologically differentiated into some cell types. The fluorescence IHC and TRAP staining techniques demonstrated these cells were detected as osteoclasts and macrophages. Furthermore, GFP-positive cells gathered adjacent blood vessels. The data suggest that GFP-positive bone marrow-derived cell migrate into periodontal tissues and differentiate periodontal tissue component-cells.

AB - We examined the transplanted bone marrow-derived cell migration into periodontal tissues. Bone marrow cells from green fluorescent protein (GFP) transgenic mice were transplanted. The immunohistochemistry (IHC) revealed that GFP-positive cells were detected in the periodontal tissues. The GFP-positive cells histopathologically differentiated into some cell types. The fluorescence IHC and TRAP staining techniques demonstrated these cells were detected as osteoclasts and macrophages. Furthermore, GFP-positive cells gathered adjacent blood vessels. The data suggest that GFP-positive bone marrow-derived cell migrate into periodontal tissues and differentiate periodontal tissue component-cells.

KW - Bone marrow-derived cells

KW - Fibroblasts

KW - Green fluorescent protein (GFP)

KW - Macrophages

KW - Osteoclasts

KW - Periodontal tissues

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Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation

Abstract

Keywords

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