Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes

Naoya Kobayashi; Masahiro Miyazaki; Kenichi Fukaya; Yusuke Inoue; Masakiyo Sakaguchi; Hirofumi Noguchi; Toshihisa Matsumura; Takamasa Watanabe; Toshinori Totsugawa; Noriaki Tanaka; Masayoshi Namba

doi:10.1177/096368970000900524

Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes

Naoya Kobayashi, Masahiro Miyazaki, Kenichi Fukaya, Yusuke Inoue, Masakiyo Sakaguchi, Hirofumi Noguchi, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Noriaki Tanaka, Masayoshi Namba

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.

Original language	English
Pages (from-to)	733-735
Number of pages	3
Journal	Cell Transplantation
Volume	9
Issue number	5
DOIs	https://doi.org/10.1177/096368970000900524
Publication status	Published - 2000

Keywords

Acute liver failure
Hepatocyte transplantation
Immoralized human hepatocytes
Simian virus 40 large T antigen

ASJC Scopus subject areas

Biomedical Engineering
Cell Biology
Transplantation

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10.1177/096368970000900524

Cite this

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title = "Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes",

abstract = "Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.",

keywords = "Acute liver failure, Hepatocyte transplantation, Immoralized human hepatocytes, Simian virus 40 large T antigen",

author = "Naoya Kobayashi and Masahiro Miyazaki and Kenichi Fukaya and Yusuke Inoue and Masakiyo Sakaguchi and Hirofumi Noguchi and Toshihisa Matsumura and Takamasa Watanabe and Toshinori Totsugawa and Noriaki Tanaka and Masayoshi Namba",

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T1 - Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes

AU - Kobayashi, Naoya

AU - Miyazaki, Masahiro

AU - Fukaya, Kenichi

AU - Inoue, Yusuke

AU - Sakaguchi, Masakiyo

AU - Noguchi, Hirofumi

AU - Matsumura, Toshihisa

AU - Watanabe, Takamasa

AU - Totsugawa, Toshinori

AU - Tanaka, Noriaki

AU - Namba, Masayoshi

PY - 2000

Y1 - 2000

N2 - Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.

AB - Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.

KW - Acute liver failure

KW - Hepatocyte transplantation

KW - Immoralized human hepatocytes

KW - Simian virus 40 large T antigen

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Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes

Abstract

Keywords

ASJC Scopus subject areas

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