Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes

Takeshi Hano; Yuji Oshima; Sang Gyoon Kim; Hina Satone; Yumi Oba; Takeshi Kitano; Suguru Inoue; Yohei Shimasaki; Tsuneo Honjo

doi:10.1016/j.chemosphere.2007.05.093

Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes

Takeshi Hano, Yuji Oshima, Sang Gyoon Kim, Hina Satone, Yumi Oba, Takeshi Kitano, Suguru Inoue, Yohei Shimasaki, Tsuneo Honjo

Graduate School of Natural Science and Technology

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).

Original language	English
Pages (from-to)	927-933
Number of pages	7
Journal	Chemosphere
Volume	69
Issue number	6
DOIs	https://doi.org/10.1016/j.chemosphere.2007.05.093
Publication status	Published - Oct 1 2007

Keywords

Endocrine disruption
Medaka
Nanoinjection
Tributyltin

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
Chemistry(all)
Pollution
Health, Toxicology and Mutagenesis

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10.1016/j.chemosphere.2007.05.093

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title = "Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes",

abstract = "We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).",

keywords = "Endocrine disruption, Medaka, Nanoinjection, Tributyltin",

author = "Takeshi Hano and Yuji Oshima and Kim, {Sang Gyoon} and Hina Satone and Yumi Oba and Takeshi Kitano and Suguru Inoue and Yohei Shimasaki and Tsuneo Honjo",

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doi = "10.1016/j.chemosphere.2007.05.093",

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AU - Hano, Takeshi

AU - Oshima, Yuji

AU - Kim, Sang Gyoon

AU - Satone, Hina

AU - Oba, Yumi

AU - Kitano, Takeshi

AU - Inoue, Suguru

AU - Shimasaki, Yohei

AU - Honjo, Tsuneo

PY - 2007/10/1

Y1 - 2007/10/1

N2 - We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).

AB - We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).

KW - Endocrine disruption

KW - Medaka

KW - Nanoinjection

KW - Tributyltin

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Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes

Abstract

Keywords

ASJC Scopus subject areas

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