Tumor antigen occurs in N-glycan of royal jelly glycoproteins: Honeybee cells synthesize T-antigen unit in N-glycan moiety

Yoshinobu Kimura; Takayuki Ushijima; Megumi Maeda; Yoichiro Hama; Mariko Kimura; Kiyoshi Okihara; Hiroyuki Sugimoto; Hideo Yamada

doi:10.1271/bbb.60331

Tumor antigen occurs in N-glycan of royal jelly glycoproteins: Honeybee cells synthesize T-antigen unit in N-glycan moiety

Yoshinobu Kimura, Takayuki Ushijima, Megumi Maeda, Yoichiro Hama, Mariko Kimura, Kiyoshi Okihara, Hiroyuki Sugimoto, Hideo Yamada

School of Agriculture

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing β1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galβ1-3GalNAcβ1-4GlcNAcβ1- 2Manα1-6 (Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-3) Manα1-4GlcNAcβ1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.

Original language	English
Pages (from-to)	2583-2587
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	70
Issue number	10
DOIs	https://doi.org/10.1271/bbb.60331
Publication status	Published - 2006

Keywords

Apis mellifera
Insect N-glycan
Royal jelly
T-antigen
Tumor marker

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.60331

Cite this

@article{00cec52bded4497cacb6caf213170b78,

title = "Tumor antigen occurs in N-glycan of royal jelly glycoproteins: Honeybee cells synthesize T-antigen unit in N-glycan moiety",

abstract = "In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing β1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galβ1-3GalNAcβ1-4GlcNAcβ1- 2Manα1-6 (Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-3) Manα1-4GlcNAcβ1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.",

keywords = "Apis mellifera, Insect N-glycan, Royal jelly, T-antigen, Tumor marker",

author = "Yoshinobu Kimura and Takayuki Ushijima and Megumi Maeda and Yoichiro Hama and Mariko Kimura and Kiyoshi Okihara and Hiroyuki Sugimoto and Hideo Yamada",

note = "Funding Information: The authors are very grateful to Dr. Kazuhito Fujiyama and Mr. Hiroyuki Kajiura (International Center of Biotechnology, Osaka University) for the generous gift of β-N-acetylgalactosamindase. We also thank the IS-MS laboratory and the SC-NMR Laboratory of Okayama University. This work was supported in part by grants from the Ministry of Education, Science, Sports, and Culture of Japan (Basic Research C, No. 17580300).",

year = "2006",

doi = "10.1271/bbb.60331",

language = "English",

volume = "70",

pages = "2583--2587",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "10",

}

TY - JOUR

T1 - Tumor antigen occurs in N-glycan of royal jelly glycoproteins

T2 - Honeybee cells synthesize T-antigen unit in N-glycan moiety

AU - Kimura, Yoshinobu

AU - Ushijima, Takayuki

AU - Maeda, Megumi

AU - Hama, Yoichiro

AU - Kimura, Mariko

AU - Okihara, Kiyoshi

AU - Sugimoto, Hiroyuki

AU - Yamada, Hideo

N1 - Funding Information: The authors are very grateful to Dr. Kazuhito Fujiyama and Mr. Hiroyuki Kajiura (International Center of Biotechnology, Osaka University) for the generous gift of β-N-acetylgalactosamindase. We also thank the IS-MS laboratory and the SC-NMR Laboratory of Okayama University. This work was supported in part by grants from the Ministry of Education, Science, Sports, and Culture of Japan (Basic Research C, No. 17580300).

PY - 2006

Y1 - 2006

N2 - In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing β1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galβ1-3GalNAcβ1-4GlcNAcβ1- 2Manα1-6 (Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-3) Manα1-4GlcNAcβ1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.

AB - In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing β1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galβ1-3GalNAcβ1-4GlcNAcβ1- 2Manα1-6 (Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-3) Manα1-4GlcNAcβ1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.

KW - Apis mellifera

KW - Insect N-glycan

KW - Royal jelly

KW - T-antigen

KW - Tumor marker

UR - http://www.scopus.com/inward/record.url?scp=33750475586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750475586&partnerID=8YFLogxK

U2 - 10.1271/bbb.60331

DO - 10.1271/bbb.60331

M3 - Article

C2 - 17031027

AN - SCOPUS:33750475586

SN - 0916-8451

VL - 70

SP - 2583

EP - 2587

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 10

ER -

Tumor antigen occurs in N-glycan of royal jelly glycoproteins: Honeybee cells synthesize T-antigen unit in N-glycan moiety

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this