Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate

Nanako Sumi; Shota Nagahiro; Eiji Nakata; Kazunori Watanabe; Takashi Ohtsuki

doi:10.1016/j.bmcl.2022.128767

Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate

Nanako Sumi, Shota Nagahiro, Eiji Nakata, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Tat-U1A-rose bengal conjugate (TatU1A-RB) was prepared as an ultrasound-sensitive RNA carrier molecule. This molecule consists of Tat cell-penetrating peptide, U1A RNA-binding protein, and rose bengal as a sonosensitizer. We demonstrated that TatU1A-RB delivered RNA via the endocytosis pathway, which was followed by ultrasound-dependent endosomal escape and cytosolic dispersion of the RNA. A short hairpin RNA (shRNA) delivered by TatU1A-RB mediated RNA interference (RNAi) ultrasound-dependently. Even by ultrasound irradiation through blood cells, RNAi could be induced with TatU1A-RB and the shRNA. This ultrasound-dependent cytosolic RNA delivery method will serve as the basis for a new approach to nucleic acid therapeutics.

Original language	English
Article number	128767
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	68
DOIs	https://doi.org/10.1016/j.bmcl.2022.128767
Publication status	Published - Jul 15 2022

Keywords

RNA delivery
RNAi
Rose Bengal
Sonosensitizer
Ultrasound

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2022.128767

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title = "Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate",

abstract = "Tat-U1A-rose bengal conjugate (TatU1A-RB) was prepared as an ultrasound-sensitive RNA carrier molecule. This molecule consists of Tat cell-penetrating peptide, U1A RNA-binding protein, and rose bengal as a sonosensitizer. We demonstrated that TatU1A-RB delivered RNA via the endocytosis pathway, which was followed by ultrasound-dependent endosomal escape and cytosolic dispersion of the RNA. A short hairpin RNA (shRNA) delivered by TatU1A-RB mediated RNA interference (RNAi) ultrasound-dependently. Even by ultrasound irradiation through blood cells, RNAi could be induced with TatU1A-RB and the shRNA. This ultrasound-dependent cytosolic RNA delivery method will serve as the basis for a new approach to nucleic acid therapeutics.",

keywords = "RNA delivery, RNAi, Rose Bengal, Sonosensitizer, Ultrasound",

author = "Nanako Sumi and Shota Nagahiro and Eiji Nakata and Kazunori Watanabe and Takashi Ohtsuki",

note = "Funding Information: This work was supported by the “Joint Usage/Research Program on Zero-Emission Energy Research”, Institute of Advanced Energy, Kyoto University, Japan (ZE2021B-21). Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

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doi = "10.1016/j.bmcl.2022.128767",

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AU - Sumi, Nanako

AU - Nagahiro, Shota

AU - Nakata, Eiji

AU - Watanabe, Kazunori

AU - Ohtsuki, Takashi

N1 - Funding Information: This work was supported by the “Joint Usage/Research Program on Zero-Emission Energy Research”, Institute of Advanced Energy, Kyoto University, Japan (ZE2021B-21). Publisher Copyright: © 2022 Elsevier Ltd

PY - 2022/7/15

Y1 - 2022/7/15

N2 - Tat-U1A-rose bengal conjugate (TatU1A-RB) was prepared as an ultrasound-sensitive RNA carrier molecule. This molecule consists of Tat cell-penetrating peptide, U1A RNA-binding protein, and rose bengal as a sonosensitizer. We demonstrated that TatU1A-RB delivered RNA via the endocytosis pathway, which was followed by ultrasound-dependent endosomal escape and cytosolic dispersion of the RNA. A short hairpin RNA (shRNA) delivered by TatU1A-RB mediated RNA interference (RNAi) ultrasound-dependently. Even by ultrasound irradiation through blood cells, RNAi could be induced with TatU1A-RB and the shRNA. This ultrasound-dependent cytosolic RNA delivery method will serve as the basis for a new approach to nucleic acid therapeutics.

AB - Tat-U1A-rose bengal conjugate (TatU1A-RB) was prepared as an ultrasound-sensitive RNA carrier molecule. This molecule consists of Tat cell-penetrating peptide, U1A RNA-binding protein, and rose bengal as a sonosensitizer. We demonstrated that TatU1A-RB delivered RNA via the endocytosis pathway, which was followed by ultrasound-dependent endosomal escape and cytosolic dispersion of the RNA. A short hairpin RNA (shRNA) delivered by TatU1A-RB mediated RNA interference (RNAi) ultrasound-dependently. Even by ultrasound irradiation through blood cells, RNAi could be induced with TatU1A-RB and the shRNA. This ultrasound-dependent cytosolic RNA delivery method will serve as the basis for a new approach to nucleic acid therapeutics.

KW - RNA delivery

KW - RNAi

KW - Rose Bengal

KW - Sonosensitizer

KW - Ultrasound

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Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate

Abstract

Keywords

ASJC Scopus subject areas

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