Ultrastructural analysis of an enterolith composed of Deoxycholic acid

Masaya Iwamuro, Yuichi Miyashima, Takahiro Yoshioka, Toshihiro Murata, Yoshio Miyabe, Yoshinari Kawai, Haruo Urata, Hidenori Shiraha, Hiroyuki Okada, Kazuhide Yamamoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


A 67-year-old Japanese man underwent enterotomy because of enterolith ileus. Component analysis by infrared spectroscopy revealed that the enterolith was composed of a high concentration of deoxycholic acid. We further analyzed and compared the ultrastructure of the enterolith and a commercially available powdered form of deoxycholic acid by means of scanning electron microscopy and energy dispersive X-ray spectroscopy. Energy dispersive X-ray spectroscopy analysis revealed that the ratios of carbon and oxygen in the enterolith were equal to those in the deoxycholic acid powder. Scanning electron microscopy analysis showed rectangular prism-shaped particles on the surface of the enterolith. This structure was similar to that of the deoxycholic acid powder. The surgically removed enterolith had a twisted and coiled appearance. Possible mechanisms underlying the formation of this unique form are discussed.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalActa medica Okayama
Issue number6
Publication statusPublished - 2014


  • Deoxycholic acid
  • Energy dispersive X-ray spectroscopy
  • Enterolith
  • Infrared spectroscopy
  • Scanning electron microscopy

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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