Ultratrace analysis based on Hadamard transform capillary electrophoresis

Takashi Kaneta, Kazuki Kosai, Totaro Imasaka

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


Hadamard transform capillary electrophoresis, which is based on a multiple sample injection technique, was combined with laser-induced fluorometry and utilized in the determination of analytes at subpicomolar levels. The sensitivity was substantially improved by increasing the order, i.e., the number of elements, of the Hadamard matrix. In fact, the signal-to-noise ratio was enhanced 18-fold by the use of a matrix of order 2047. A feasibility study was carried out by computer simulation to study the detection of an average of less than a single molecule in a single injection volume. The signal peak was clearly observable even under conditions at which only 0.3 molecule is present in the volume. Thus, this approach is potentially useful for ultratrace analysis, in which conventional single-injection capillary electrophoresis cannot be applied.

Original languageEnglish
Pages (from-to)2257-2260
Number of pages4
JournalAnalytical Chemistry
Issue number10
Publication statusPublished - May 15 2002
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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