Up-regulation of integrin β3 expression by cyclic stretch in human umbilical endothelial cells

Masahiko Suzuki; Keiji Naruse; Yoshihiro Asano; Tetsuya Okamoto; Naomichi Nishikimi; Tsunehisa Sakurai; Yuji Nimura; Masahiro Sokabe

doi:10.1006/bbrc.1997.7364

Up-regulation of integrin β₃ expression by cyclic stretch in human umbilical endothelial cells

Masahiko Suzuki, Keiji Naruse, Yoshihiro Asano, Tetsuya Okamoto, Naomichi Nishikimi, Tsunehisa Sakurai, Yuji Nimura, Masahiro Sokabe

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

The effect of uni-axial cyclic mechanical stretch on the expression of the adhesion protein integrin was investigated. Human umbilical endothelial cells (HUVECs) cultured on fibronectin coated silicon membranes were subjected to uni-axial cyclic stretch. The level of expression of integrin β₃ mRNA was found to be increased and peaked at 4 hours in response to cyclic stretch using a semiquantitative RT-PCR method. The increased level of the integrin mRNA from stretched HUVECs remained higher than that from non-stretched controls. The amount of integrin β₃ also increased and peaked at 12 hr. Immuno-fluorescent microscopy revealed that the amount of integrin β₃ adhesions increased in stretched HUVECs compared with that in non-stretched HUVECs. These results suggest that uni-axial cyclic stretch up-regulates the expression of integrin β₃. This increase in integrin β₃ may enhance the adhesiveness to the substratum and contribute to the protection of HUVECs against being peeled off from the vessel wall.

Original language	English
Pages (from-to)	372-376
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	239
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1997.7364
Publication status	Published - Oct 20 1997
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1997.7364

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title = "Up-regulation of integrin β3 expression by cyclic stretch in human umbilical endothelial cells",

abstract = "The effect of uni-axial cyclic mechanical stretch on the expression of the adhesion protein integrin was investigated. Human umbilical endothelial cells (HUVECs) cultured on fibronectin coated silicon membranes were subjected to uni-axial cyclic stretch. The level of expression of integrin β3 mRNA was found to be increased and peaked at 4 hours in response to cyclic stretch using a semiquantitative RT-PCR method. The increased level of the integrin mRNA from stretched HUVECs remained higher than that from non-stretched controls. The amount of integrin β3 also increased and peaked at 12 hr. Immuno-fluorescent microscopy revealed that the amount of integrin β3 adhesions increased in stretched HUVECs compared with that in non-stretched HUVECs. These results suggest that uni-axial cyclic stretch up-regulates the expression of integrin β3. This increase in integrin β3 may enhance the adhesiveness to the substratum and contribute to the protection of HUVECs against being peeled off from the vessel wall.",

author = "Masahiko Suzuki and Keiji Naruse and Yoshihiro Asano and Tetsuya Okamoto and Naomichi Nishikimi and Tsunehisa Sakurai and Yuji Nimura and Masahiro Sokabe",

note = "Funding Information: We thank Drs. Shibata, Narita, and Hoshigaoka Maternity Clinic, Nagoya, Japan for providing human umbilical cords. We also thank T. Murakami and M. Takahashi for excellent technical assistance. This work was supported by a Grant-in Aid from the Ministry of Education Science and Culture (K.N., M.S.) Japan, and by Nitto Foundation (K.N.).",

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AU - Suzuki, Masahiko

AU - Naruse, Keiji

AU - Asano, Yoshihiro

AU - Okamoto, Tetsuya

AU - Nishikimi, Naomichi

AU - Sakurai, Tsunehisa

AU - Nimura, Yuji

AU - Sokabe, Masahiro

N1 - Funding Information: We thank Drs. Shibata, Narita, and Hoshigaoka Maternity Clinic, Nagoya, Japan for providing human umbilical cords. We also thank T. Murakami and M. Takahashi for excellent technical assistance. This work was supported by a Grant-in Aid from the Ministry of Education Science and Culture (K.N., M.S.) Japan, and by Nitto Foundation (K.N.).

PY - 1997/10/20

Y1 - 1997/10/20

N2 - The effect of uni-axial cyclic mechanical stretch on the expression of the adhesion protein integrin was investigated. Human umbilical endothelial cells (HUVECs) cultured on fibronectin coated silicon membranes were subjected to uni-axial cyclic stretch. The level of expression of integrin β3 mRNA was found to be increased and peaked at 4 hours in response to cyclic stretch using a semiquantitative RT-PCR method. The increased level of the integrin mRNA from stretched HUVECs remained higher than that from non-stretched controls. The amount of integrin β3 also increased and peaked at 12 hr. Immuno-fluorescent microscopy revealed that the amount of integrin β3 adhesions increased in stretched HUVECs compared with that in non-stretched HUVECs. These results suggest that uni-axial cyclic stretch up-regulates the expression of integrin β3. This increase in integrin β3 may enhance the adhesiveness to the substratum and contribute to the protection of HUVECs against being peeled off from the vessel wall.

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Up-regulation of integrin β₃ expression by cyclic stretch in human umbilical endothelial cells

Abstract

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