Uptake of histamine by mouse peritoneal macrophages and a macrophage cell line, RAW264.7

Satoshi Tanaka, Katsuya Deai, Mariko Inagaki, Atsushi Ichikawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We have previously demonstrated that dietary histamine is accumulated in the spleens of L-histidine decarboxylase (HDC)-deficient mice, which lack endogenous histamine synthesis. To characterize the clearance system for dietary histamine in mice, we investigated the cell type and mechanism responsible for histamine uptake in the spleens of HDC-deficient mice. Immunohistochemical analyses using an antihistamine antibody indicated that a portion of the CD14+ cells in the spleen is involved in histamine storage. Peritoneal macrophages obtained from Balb/c mice and a mouse macrophage cell line, RAW264.7, had potential for histamine uptake, which was characterized by a low affinity and high capacity for histamine. The histamine uptake by RAW264.7 cells was observed at physiological temperature and was potently inhibited by pyrilamine, chlorpromazine, quinidine, and chloroquine, moderately inhibited by Nα-methylhistamine, dopamine, and serotonin, and not affected by tetraethylammonium and 1-methyl-4-phenylpyridinium. Intracellular histamine was not metabolized in RAW264.7 cells and was released at physiological temperature in the absence of extracellular histamine. These results suggest that histamine uptake by macrophages may be involved in the clearance of histamine in the local histamine-enriched environment.

Original languageEnglish
Pages (from-to)C592-C598
JournalAmerican Journal of Physiology - Cell Physiology
Issue number3 54-3
Publication statusPublished - Sept 1 2003


  • Cation transporter
  • Chlorpromazine
  • Pyrilamine
  • Quinidine

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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