Variant alpha and beta biodiversity of the genus in dadiah through deep sequencing 16S Ribosomonal RNA genes

Dadiah is a traditional spontaneous fermentation of buffalo milk from West Sumatra that doesn't go through pasteurized stages. The aim of the study is to find out the biodiversity of α and β diversity of the microbiota in the level of the genus in dadiah. The V3 -V4 hypervariable region of the 16 S rRNA deep sequencing was used to the detected genus of bacteria. The available data were analyzed by Alpha Diversity (richness and evenness) and Beta Diversity (Taxonomy Chart Heatmaps). These studies revealed that the most divergent sample is appropriate to C3 with Shanon diversity Index 2.014; meanwhile, the most evenness fit in to B4 with the Simpson diversity index of 0.573. Further, the highest value of the heatmap taxonomic chart on the B4 sample has its place in Lactococcus. There were differences in dadiah microbiota composition among the samples based on different areas. Each sample was found Lactococcus and Lactobacillus with the largest populations.