TY - JOUR
T1 - Variations in the viral genome and biological properties of bovine leukemia virus wild-type strains
AU - Murakami, Hironobu
AU - Uchiyama, Jumpei
AU - Suzuki, Chihiro
AU - Nikaido, Sae
AU - Shibuya, Kaho
AU - Sato, Reiichiro
AU - Maeda, Yosuke
AU - Tomioka, Michiko
AU - Takeshima, Shin nosuke
AU - Kato, Hajime
AU - Sakaguchi, Masahiro
AU - Sentsui, Hiroshi
AU - Aida, Yoko
AU - Tsukamoto, Kenji
N1 - Funding Information:
This work was partially supported by a research project grant awarded by the Azabu University Research Services Division and JSPS KAKENHI Grant Number 17K15385 .
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/7/15
Y1 - 2018/7/15
N2 - Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis (EBL), which causes enormous economic losses in the livestock industry worldwide. To reduce the economic loss caused by BLV infection, it is important to clarify the characters associated with BLV transmissibility and pathogenesis in cattle. In this study, we focused on viral characters and examined spontaneous mutations in the virus and viral properties by analyses of whole genome sequences and BLV molecular clones derived from cows with and without EBL. Genomic analysis indicated that all 28 strains harbored limited genetic variations but no deletion mutations that allowed classification into three groups (A, B, and C), except for one strain. Some nucleotide/amino acid substitutions were specific to a particular group. On the other hand, these genetic variations were not associated with the host bovine leukocyte antigen-DRB3 allele, which is known to be related to BLV pathogenesis. The viral replication activity in vitro was high, moderate, and low in groups A, B, and C, respectively. In addition, the proviral load, which is related to BLV transmissibility and pathogenesis, was high in cows infected with group A strains and low in those infected with group B/C strains. Therefore, these results suggest that limited genetic variations could affect viral properties relating to BLV transmissibility and pathogenesis.
AB - Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis (EBL), which causes enormous economic losses in the livestock industry worldwide. To reduce the economic loss caused by BLV infection, it is important to clarify the characters associated with BLV transmissibility and pathogenesis in cattle. In this study, we focused on viral characters and examined spontaneous mutations in the virus and viral properties by analyses of whole genome sequences and BLV molecular clones derived from cows with and without EBL. Genomic analysis indicated that all 28 strains harbored limited genetic variations but no deletion mutations that allowed classification into three groups (A, B, and C), except for one strain. Some nucleotide/amino acid substitutions were specific to a particular group. On the other hand, these genetic variations were not associated with the host bovine leukocyte antigen-DRB3 allele, which is known to be related to BLV pathogenesis. The viral replication activity in vitro was high, moderate, and low in groups A, B, and C, respectively. In addition, the proviral load, which is related to BLV transmissibility and pathogenesis, was high in cows infected with group A strains and low in those infected with group B/C strains. Therefore, these results suggest that limited genetic variations could affect viral properties relating to BLV transmissibility and pathogenesis.
KW - Bovine leukemia virus
KW - Genetic variation
KW - Molecular clone
KW - Phylogenetic analysis
KW - Proviral load
KW - Viral property
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U2 - 10.1016/j.virusres.2018.06.005
DO - 10.1016/j.virusres.2018.06.005
M3 - Article
C2 - 29913249
AN - SCOPUS:85048720923
SN - 0168-1702
VL - 253
SP - 103
EP - 111
JO - Virus Research
JF - Virus Research
ER -
