TY - GEN
T1 - Varietal variation in microsatellite and caps for ethylene-related genes and its possible association with agronomic characters in melon (cucumis melo)
AU - Akashi, Y.
AU - Ezura, H.
AU - Kubo, Y.
AU - Masuda, M.
AU - Kato, K.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Shelf-life of fruit is one of the most important traits in melon breeding. Shelf-life of climacteric fruits is closely related with ethylene whose biosynthesis is controlled by ACC synthase and ACC oxidase genes. As the first step to develop selection markers for shelf-life in melon (Cucumis melo), microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms were analyzed in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), resulting in the establishment of six microsatellite and two CAPS markers. Five markers were developed in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeat of (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n was also detected in CMe-ACS2. CAPS and microsatellite markers specific to vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.
AB - Shelf-life of fruit is one of the most important traits in melon breeding. Shelf-life of climacteric fruits is closely related with ethylene whose biosynthesis is controlled by ACC synthase and ACC oxidase genes. As the first step to develop selection markers for shelf-life in melon (Cucumis melo), microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms were analyzed in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), resulting in the establishment of six microsatellite and two CAPS markers. Five markers were developed in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeat of (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n was also detected in CMe-ACS2. CAPS and microsatellite markers specific to vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.
KW - Phylogeny
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U2 - 10.17660/ActaHortic.2002.588.50
DO - 10.17660/ActaHortic.2002.588.50
M3 - Conference contribution
AN - SCOPUS:84879647983
SN - 9789066057760
T3 - Acta Horticulturae
SP - 313
EP - 316
BT - II International Symposium on Cucurbits
PB - International Society for Horticultural Science
ER -