Various molecular species of diacylglycerol hydroperoxide activate human neutrophils via PKC activation

Yasuhiro Kambayashi, Susumu Takekoshi, Yutaka Tanino, Keiichi Watanabe, Minoru Nakano, Yoshiaki Hitomi, Tomoko Takigawa, Keiki Ogino, Yorihiro Yamamoto

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


We have proposed that diacylglycerol hydroperoxide-induced unregulated signal transduction causes oxidative stress-related diseases. In this study, we investigated which molecular species of diacylglycerol hydroperoxide activated human peripheral neutrophils. All diacylglycerol hydroperoxides, diacylglycerol hydroxides, and diacyglycerols tested in the present study induced superoxide production by neutrophils. The ability to activate neutrophils among molecular species containing the same fatty acid composition was as follows; diacylglycerol hydroperoxide>diacylglycerol hydroxide≥diacylglycerol. The diacylglycerol hydroperoxide composed of linoleate was a stronger activator for neutrophils than that composed of arachidonate. 1-Palmitoyl-2-linoleoylglycerol hydroperoxide (PLG-OOH) was the strongest stimulator for neutrophils. We reconfirmed that PLG-OOH activated protein kinase C (PKC) in neutrophils. PLG-OOH induced the phosphorylation of p47phox, a substrate of PKC and a cytosolic component of NADPH oxidase, in neutrophils, as did N-formyl-methionyl-leucyl-phenylalanine or 4β-phorbol-12β-myristate- 13α-acetate. Moreover, the time course of p47phox phosphorylation was comparable to that of superoxide production. These results suggest that PLG-OOH activated intracellular protein kinase C. PLG-OOH, produced via an uncontrolled process, can act as a biological second messenger to cause inflammatory disease from oxidative stress.

Original languageEnglish
Pages (from-to)68-75
Number of pages8
Journaljournal of clinical biochemistry and nutrition
Issue number1
Publication statusPublished - Jul 2007
Externally publishedYes


  • 1-Palmitoyl-2-linoleoylglycerol hydroperoxide
  • Neutrophil
  • Oxidative stress
  • Protein kinase C
  • p47

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry


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