TY - JOUR
T1 - Vimentin binds IRAP and is involved in GLUT4 vesicle trafficking
AU - Hirata, Yohko
AU - Hosaka, Toshio
AU - Iwata, Takeo
AU - Le, Chung T.K.
AU - Jambaldorj, Bayasgalan
AU - Teshigawara, Kiyoshi
AU - Harada, Nagakatsu
AU - Sakaue, Hiroshi
AU - Sakai, Tohru
AU - Yoshimoto, Katsuhiko
AU - Nakaya, Yutaka
N1 - Funding Information:
We would like to thank Dr. Masashi Yoshida, Takaaki Shimohata and Haruka Yonemoto, the University of Tokushima and Dr. Paul F. Pilch and Michael Breen, Boston University School of Medicine for valuable advice. This work was supported by a Grant-in-Aid for Scientific Research 21500685 (to T. H.) from the Ministry of Education, Science, and Culture of Japan .
PY - 2011/2/4
Y1 - 2011/2/4
N2 - Insulin-responsive aminopeptidase (IRAP) and GLUT4 are two major cargo proteins of GLUT4 storage vesicles (GSVs) that are translocated from a postendosomal storage compartment to the plasma membrane (PM) in response to insulin. The cytoplasmic region of IRAP is reportedly involved in retention of GSVs. In this study, vimentin was identified using the cytoplasmic domain of IRAP as bait. The validity of this interaction was confirmed by pull-down assays and immunoprecipitation in 3T3-L1 adipocytes. In addition, it was shown that GLUT4 translocation to the PM by insulin was decreased in vimentin-depleted adipocytes, presumably due to dispersing GSVs away from the cytoskeleton. These findings suggest that the IRAP binding protein, vimentin, plays an important role in retention of GSVs.
AB - Insulin-responsive aminopeptidase (IRAP) and GLUT4 are two major cargo proteins of GLUT4 storage vesicles (GSVs) that are translocated from a postendosomal storage compartment to the plasma membrane (PM) in response to insulin. The cytoplasmic region of IRAP is reportedly involved in retention of GSVs. In this study, vimentin was identified using the cytoplasmic domain of IRAP as bait. The validity of this interaction was confirmed by pull-down assays and immunoprecipitation in 3T3-L1 adipocytes. In addition, it was shown that GLUT4 translocation to the PM by insulin was decreased in vimentin-depleted adipocytes, presumably due to dispersing GSVs away from the cytoskeleton. These findings suggest that the IRAP binding protein, vimentin, plays an important role in retention of GSVs.
KW - GLUT4
KW - IRAP
KW - Vimentin
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U2 - 10.1016/j.bbrc.2010.12.134
DO - 10.1016/j.bbrc.2010.12.134
M3 - Article
C2 - 21216232
AN - SCOPUS:79551484024
SN - 0006-291X
VL - 405
SP - 96
EP - 101
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -