Virtual phase conjugation based optical tomography for single-shot three-dimensional imaging

Yuta Goto, Atsushi Okamoto, Atsushi Shibukawa, Kazuhisa Ogawa, Akihisa Tomita

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


We propose a virtual phase conjugation (VPC) based optical tomography (VPC-OT) for realizing single-shot optical tomographic imaging systems. Using a computer-based numerical beam propagation, the VPC combines pre-modulation and post-demodulation of the probe beam’s wavefront, which provides an optical sectioning capability for resolving the depth coordinates. In VPC-OT, the physical optical microscope system and VPC are coupled using digital holography. Therefore, in contrast to conventional optical tomographic imaging (OTI) systems, this method does not require additional elements such as low-coherence light sources or confocal pinholes. It is challenging to obtain single-shot three-dimensional (3D) tomographic images using a conventional OTI system; however, this can be achieved using VPC-OT, which employs both digital holography and computer based numerical beam propagation. In addition, taking into account that VPC-OT is based on a complex amplitude detection using digital holography, this method allows us to simultaneously obtain quantitative phase contrast images. Using an objective lens with a numerical aperture (NA) of 0.8, we demonstrate a single-shot 3D imaging of frog blood cells with a depth resolution of 0.94 µm.

Original languageEnglish
Pages (from-to)3779-3790
Number of pages12
JournalOptics Express
Issue number4
Publication statusPublished - Feb 19 2018
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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