Visible light-induced crosslinkable gelatin

Tae Il Son; Makoto Sakuragi; Sawa Takahashi; Sei Obuse; Jeonghwa Kang; Masako Fujishiro; Haruhiko Matsushita; Jiansheng Gong; Shigeru Shimizu; Yusuke Tajima; Yasuhiro Yoshida; Kazuomi Suzuki; Toshio Yamamoto; Mariko Nakamura; Yoshihiro Ito

doi:10.1016/j.actbio.2010.05.018

Visible light-induced crosslinkable gelatin

Tae Il Son, Makoto Sakuragi, Sawa Takahashi, Sei Obuse, Jeonghwa Kang, Masako Fujishiro, Haruhiko Matsushita, Jiansheng Gong, Shigeru Shimizu, Yusuke Tajima, Yasuhiro Yoshida, Kazuomi Suzuki, Toshio Yamamoto, Mariko Nakamura, Yoshihiro Ito

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, which is a visible light sensitizer. Irradiation by visible light solidified the aqueous solution. In addition, when the solution was cast on a plate, dried and photo-irradiated in the presence of a photomask a micropattern was formed that matched the micropattern on the photomask. The gelatin-immobilized regions enhanced cell adhesion. It was also confirmed that the gelatin incorporating furfuryl and Rose Bengal have no significant toxicity. The photo-crosslinkable gelatin was employed as a direct pulp capping material in the dental field. Considering these results, this system could be useful as a new type of visible light-induced crosslinkable biosealant.

Original language	English
Pages (from-to)	4005-4010
Number of pages	6
Journal	Acta Biomaterialia
Volume	6
Issue number	10
DOIs	https://doi.org/10.1016/j.actbio.2010.05.018
Publication status	Published - Oct 2010
Externally published	Yes

Keywords

Biosealant
Direct pulp capping
Gelatin
Photo-crosslinking
Visible light

ASJC Scopus subject areas

Biotechnology
Biomaterials
Biochemistry
Biomedical Engineering
Molecular Biology

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10.1016/j.actbio.2010.05.018

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title = "Visible light-induced crosslinkable gelatin",

abstract = "A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, which is a visible light sensitizer. Irradiation by visible light solidified the aqueous solution. In addition, when the solution was cast on a plate, dried and photo-irradiated in the presence of a photomask a micropattern was formed that matched the micropattern on the photomask. The gelatin-immobilized regions enhanced cell adhesion. It was also confirmed that the gelatin incorporating furfuryl and Rose Bengal have no significant toxicity. The photo-crosslinkable gelatin was employed as a direct pulp capping material in the dental field. Considering these results, this system could be useful as a new type of visible light-induced crosslinkable biosealant.",

keywords = "Biosealant, Direct pulp capping, Gelatin, Photo-crosslinking, Visible light",

author = "Son, {Tae Il} and Makoto Sakuragi and Sawa Takahashi and Sei Obuse and Jeonghwa Kang and Masako Fujishiro and Haruhiko Matsushita and Jiansheng Gong and Shigeru Shimizu and Yusuke Tajima and Yasuhiro Yoshida and Kazuomi Suzuki and Toshio Yamamoto and Mariko Nakamura and Yoshihiro Ito",

year = "2010",

month = oct,

doi = "10.1016/j.actbio.2010.05.018",

language = "English",

volume = "6",

pages = "4005--4010",

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T1 - Visible light-induced crosslinkable gelatin

AU - Son, Tae Il

AU - Sakuragi, Makoto

AU - Takahashi, Sawa

AU - Obuse, Sei

AU - Kang, Jeonghwa

AU - Fujishiro, Masako

AU - Matsushita, Haruhiko

AU - Gong, Jiansheng

AU - Shimizu, Shigeru

AU - Tajima, Yusuke

AU - Yoshida, Yasuhiro

AU - Suzuki, Kazuomi

AU - Yamamoto, Toshio

AU - Nakamura, Mariko

AU - Ito, Yoshihiro

PY - 2010/10

Y1 - 2010/10

N2 - A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, which is a visible light sensitizer. Irradiation by visible light solidified the aqueous solution. In addition, when the solution was cast on a plate, dried and photo-irradiated in the presence of a photomask a micropattern was formed that matched the micropattern on the photomask. The gelatin-immobilized regions enhanced cell adhesion. It was also confirmed that the gelatin incorporating furfuryl and Rose Bengal have no significant toxicity. The photo-crosslinkable gelatin was employed as a direct pulp capping material in the dental field. Considering these results, this system could be useful as a new type of visible light-induced crosslinkable biosealant.

AB - A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, which is a visible light sensitizer. Irradiation by visible light solidified the aqueous solution. In addition, when the solution was cast on a plate, dried and photo-irradiated in the presence of a photomask a micropattern was formed that matched the micropattern on the photomask. The gelatin-immobilized regions enhanced cell adhesion. It was also confirmed that the gelatin incorporating furfuryl and Rose Bengal have no significant toxicity. The photo-crosslinkable gelatin was employed as a direct pulp capping material in the dental field. Considering these results, this system could be useful as a new type of visible light-induced crosslinkable biosealant.

KW - Biosealant

KW - Direct pulp capping

KW - Gelatin

KW - Photo-crosslinking

KW - Visible light

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Visible light-induced crosslinkable gelatin

Abstract

Keywords

ASJC Scopus subject areas

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