TY - JOUR
T1 - VopV, an F-actin-binding type III secretion effector, is required for vibrio parahaemolyticus-induced enterotoxicity
AU - Hiyoshi, Hirotaka
AU - Kodama, Toshio
AU - Saito, Kazunobu
AU - Gotoh, Kazuyoshi
AU - Matsuda, Shigeaki
AU - Akeda, Yukihiro
AU - Honda, Takeshi
AU - Iida, Tetsuya
N1 - Funding Information:
We wish to thank Ms. Hiroko Omori of the Core Instrumentation Facility (RIMD, Osaka University) for the use of a transmission electron microscope. This work was supported by Grants-in-Aid for Young Scientists and for Scientific Research on Priority Areas of Applied Genomics and Matrix of Infection Phenomena from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2011/10/4
Y1 - 2011/10/4
N2 - Vibrio parahaemolyticus, a Gram-negative halophilic bacterium that causes acute gastroenteritis in humans, is characterized by two type III secretion systems (T3SS), namely T3SS1 and T3SS2. T3SS2 is indispensable for enterotoxicity but the effector(s) involved are unknown. Here, we identify VopV as a critical effector that is required to mediate V. parahaemolyticus T3SS2-dependent enterotoxicity. VopV was found to possess multiple F-actin-binding domains and the enterotoxicity caused by VopV correlated with its F-actin-binding activity. Furthermore, a T3SS2-related secretion system and a vopV homologous gene were also involved in the enterotoxicity of a non-O1/non-O139 V. cholerae strain. These results indicate that the F-actin-targeting effector VopV is involved in enterotoxic activity of T3SS2-possessing bacterial pathogens.
AB - Vibrio parahaemolyticus, a Gram-negative halophilic bacterium that causes acute gastroenteritis in humans, is characterized by two type III secretion systems (T3SS), namely T3SS1 and T3SS2. T3SS2 is indispensable for enterotoxicity but the effector(s) involved are unknown. Here, we identify VopV as a critical effector that is required to mediate V. parahaemolyticus T3SS2-dependent enterotoxicity. VopV was found to possess multiple F-actin-binding domains and the enterotoxicity caused by VopV correlated with its F-actin-binding activity. Furthermore, a T3SS2-related secretion system and a vopV homologous gene were also involved in the enterotoxicity of a non-O1/non-O139 V. cholerae strain. These results indicate that the F-actin-targeting effector VopV is involved in enterotoxic activity of T3SS2-possessing bacterial pathogens.
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U2 - 10.1016/j.chom.2011.08.014
DO - 10.1016/j.chom.2011.08.014
M3 - Article
C2 - 22018240
AN - SCOPUS:80054915314
SN - 1931-3128
VL - 10
SP - 401
EP - 409
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 4
ER -