TY - JOUR
T1 - Wax ester production from n-alkanes by Acinetobacter sp. strain M-1
T2 - Ultrastructure of cellular inclusions and role of acyl coenzyme a reductase
AU - Ishige, Takeru
AU - Tani, Akio
AU - Takabe, Keiji
AU - Kawasaki, Kazunori
AU - Sakai, Yasuyoshi
AU - Kato, Nobuo
PY - 2002
Y1 - 2002
N2 - Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.
AB - Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.
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U2 - 10.1128/AEM.68.3.1192-1195.2002
DO - 10.1128/AEM.68.3.1192-1195.2002
M3 - Article
C2 - 11872467
AN - SCOPUS:0036194910
SN - 0099-2240
VL - 68
SP - 1192
EP - 1195
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 3
ER -