@inbook{b40a7cea04fd41f0afd5b1c4afc87fdd,
title = "Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free Fractions, and Cells",
abstract = "Cellular Communication Network (CCN) proteins are growth factors that play key roles in many pathophysiological events, including bone formation, wound healing, and cancer. CCN factors and fragments generated by metalloproteinases-dependent cleavage are often associated with extracellular matrix (ECM) or small extracellular vesicles (sEVs) such as exosomes or matrix-coated vesicles. We provide reliable methods and protocols for Western blotting to analyze CCN factors and fragments in cells, sEVs, and vesicle-free fractions.",
keywords = "CCN family proteins, CCN fragments, CTGF, Cell communication network, Exosomes, Extracellular matrix, Extracellular vesicles, Matricellular proteins, Matrix vesicles, Metalloproteinases",
author = "Kisho Ono and Yuka Okusha and Tran, \{Manh Tien\} and Koki Umemori and Takanori Eguchi",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2744-0\_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "39--57",
booktitle = "Methods in Molecular Biology",
}
