Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free Fractions, and Cells

Kisho Ono, Yuka Okusya, Manh Tien Tran, Koki Umemori, Takanori Eguchi

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Cellular Communication Network (CCN) proteins are growth factors that play key roles in many pathophysiological events, including bone formation, wound healing, and cancer. CCN factors and fragments generated by metalloproteinases-dependent cleavage are often associated with extracellular matrix (ECM) or small extracellular vesicles (sEVs) such as exosomes or matrix-coated vesicles. We provide reliable methods and protocols for Western blotting to analyze CCN factors and fragments in cells, sEVs, and vesicle-free fractions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages39-57
Number of pages19
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2582
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CCN family proteins
  • CCN fragments
  • Cell communication network
  • CTGF
  • Exosomes
  • Extracellular matrix
  • Extracellular vesicles
  • Matricellular proteins
  • Matrix vesicles
  • Metalloproteinases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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