Western blotting analysis of CCN proteins in calcified tissues

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


Western blotting is widely used for protein analysis. We routinely perform such analysis for evaluating the production levels of CCN family proteins in a variety of cells under various conditions. In this chapter, we describe our Western blotting protocol to estimate protein production profiles of CCN family members after having assessed the specificity of the antibodies against each CCN member protein to ensure no crossreaction with other CCN member proteins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Amino acid homology
  • Conserved module
  • Cross-reaction
  • Member-specific antibody
  • Redundancy of CCN members

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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