Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation

Ken Ichi Takemaru, Satoshi Harashima, Hitoshi Ueda, Susumu Hirose

Research output: Contribution to journalArticlepeer-review

91 Citations (Scopus)


Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-binding protein (TBP). The MBF1 sequence is highly conserved across species from Saccharomyces cerevisiae to human. Here we provide evidence acquired in vitro and in vivo that yeast MBF1 mediates GCN4-dependent transcriptional activation by bridging the DNA-binding region of GCN4 and TBP. These findings indicate that the coactivator MBF1 functions by recruiting TBP to promoters where DNA-binding regulators are bound.

Original languageEnglish
Pages (from-to)4971-4976
Number of pages6
JournalMolecular and Cellular Biology
Issue number9
Publication statusPublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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